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中文摘要：
为了探讨耐喹诺酮类决定区（QRDR）、外排泵负调控基因（acrR、marR和soxR）突变对临床分离株氟喹诺酮（FQs）高水平耐药的影响,本研究对临床分离的18株FQs耐药大肠杆菌（E.coli）,采用PCR方法检测QRDR、acrR、marR和soxR的突变情况;通过RT-PCR的方法检测外排泵及膜孔蛋白相关基因的表达水平。结果显示,QRDR的突变主要集中在常规突变GyrA（Ser83Leu和Asp87Asn）和ParC（Ser80Ile）,同时也检测出稀有突变ParC Glu84Gly、Glu84Lys、Glu84Val和Glu84Ala,ParE Ser458Ala等。ED28在acrR基因存在777bp插入序列;12株菌（包括ATCC25922）在MarR存在Gly103Ser和Tyr137His双突变,其中EP26和EG42存在插入片段;ED40在SoxR存在Thr38Ser、Gly74Arg氨基酸替换。在多突变药菌株中,AcrAB的表达水平明显升高,OmpC和OmpF表达量降低、甚至缺失。
英文摘要：
The objective of this study was to detect the role of QRDR, acrR, marR and soxR in genetic characterization of bighly fluoroquinolone-resistant clinical Escherichia coli strains. PCR method was used to analyse the mutations of QRDR, aerR, marR and soxR. RT-PCR method was used to detect the expression level of efflux pump and porin related genes. Muta- tions of QRDR mainly fell into GyrA（Ser83Leu and Asp87Asn） and ParC（Ser80Ile）, and other mutations had also detected, such as ParC Glu84Gly, Glu84Lys, Glu84Val and Glu84Ala,ParE Ser458Ala,et al. There was an insertion of 777 hp fragment within acrR in ED28. 12 of 18 stains including ATCC25922 had double mutations （Glyl03Ser and Tyr137His） in MarR and two （EP26 and EG42） of them had an insertion. ED40 had amino acid substitution （Thr38Ser and Gly74Arg） in SoxR. In mul- tiple mutations strains, there were markedly increased expression of AcrAB and decrease expression of porin or porin loss.
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