中文摘要：

介绍了一种基于大肠杆菌（Escherichia coli）的CellSense生物传感器毒性分析系统,对其毒性分析条件进行了初步优化,并探讨了其在重金属离子生物急性毒性分析中的应用性能.结果表明,以p-苯醌为电子传递介质时,基于大肠杆菌的CellSense生物传感器具有良好的毒性分析特性,测试得Hg^2＋、Cu^2＋、Zn^2＋和Ni^2＋四种金属离子的EC50分别为1.1、6.5、66.0和119.0μg·mL^-1;呼吸基质的pH低于6.0和盐度（NaCl）高于3%（w/w）时,对E.coli的活性产生一定的抑制作用.

英文摘要：

A cost-effective strategy using an amperometric biosensor with Escherichia coli （E. coli ） that provides a rapid toxicity determination of toxicants is described. The Cellsense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal. It is showed that CeUSense biosensor with E. coli has a good performance when p-benzoquinone is chosen as redox mediator. The 50% effective concentrations （EC50）values of Hg^2＋, Cu^2＋, Zn^2＋ and Ni^2＋ are 1.1, 6.5, 66.0 and 119.0μg.mL^-1, respectively, which are in accordance with the results of conventional bacterial counting method. When the respiratory substrates pH is lower than about 6.0 and salinity（NaCl） is higher than 3%（w/w）, the activity of E. coli is inhibited significantly.