中文摘要：

双 shelled 草鲤鱼 reovirus (GCRV ) 能够内长的 RNA 抄写并且处理。染色体顺序分析表明了蛋白质 VP2，在基因片断 2 编码了(S2 ) ，是通常认为的 RNA 依赖的 RNA 聚合酶(RdRp ) 。在以前的工作，我们表示了与 RNA 聚合酶活动被联系的 VP2 的功能的区域(作为 rVP2390900 表示了) 在 E。coli 并且对 VP2 准备了 polyclonal 抗体。描绘 GCRV RNA 聚合酶， recombinant 全身的 VP2 (rVP2 ) 首先在一个 baculovirus 系统被构造并且表示，，有一个依附的他的标签的熔化蛋白质。Immunofluorescence (如果) 试金，和 immunoblot (IB ) ，从两个的分析表示了房间摘录并且净化了 Histagged rVP2，证明 rVP2 成功地在 Sf9 房间被表示。replicase 活动的进一步的描述显示出 poly 展出的那净化的 rVP2 和 GCRV 粒子(C) 依赖者 poly (G) 聚合酶活动。RNA 酶的活动要求了二价的阳离子 Mg2+ ，并且在 28 点是最佳的 ???????????? t

英文摘要：

The double-shelled grass carp reovirus （GCRV） is capable of endogenous RNA transcription and processing.Genome sequence analysis has revealed that the protein VP2,encoded by gene segment 2 （S2）,is the putative RNA-dependent RNA polymerase （RdRp）.In previous work,we have ex-pressed the functional region of VP2 that is associated with RNA polymerase activity （denoted as rVP2390-900） in E.coil and have prepared a polyclonal antibody against VP2.To characterize the GCRV RNA polymerase,a recombinant full-length VP2 （rVP2） was first constructed and expressed in a baculovirus system,as a fusion protein with an attached His-tag.Immunofluorescence （IF） assays,together with immunoblot （IB） analyses from both expressed cell extracts and purified Histagged rVP2,showed that rVP2 was successfully expressed in Sf9 cells.Further characterization of the replicase activity showed that purified rVP2 and GCRV particles exhibited poly（C）-dependent poly（G） polymerase activity.The RNA enzymatic activity required the divalent cation Mg2＋,and was optimal at 28 ℃.The results provide a foundation for further studies on the RNA polymerases of aquareoviruses during viral transcription and replication.