中文摘要：

目的建立大鼠血浆柴胡皂苷b2检测的高效液相色谱方法。方法色谱柱为Agilent TC-C18色谱柱（150 mm×4.6 mm,5μm）,流动相为乙腈-0.2%三氟乙酸-水（40∶20∶40）,流速为1.0 mL.min-1,柱温为30℃,检测波长为254nm。以伏立康唑为内标,血浆在碱性条件下经乙酸乙酯萃取后检测。结果柴胡皂苷b2浓度在0.05～6.00 mg.L-1内线性关系良好（r=0.999 7）,定量下限为0.05 mg.L-1。低中高3个浓度（0.1,1.0,4.0 mg.L-1）的质控样品日内RSD分别为5.20%,4.80%和2.84%,日间RSD分别为6.39%,5.13%和2.47%;相对回收率分别为（97.17±7.22）%,（103.87±4.28）%和（101.75±1.54）%。结论该方法简便、快速、准确、重复性好,适用于大鼠血浆柴胡皂苷b2浓度的测定及其药代动力学研究。

英文摘要：

Objective To develop a HPLC method for the determination of saikosaponin b2 in rat plasma.Methods The analytical column was packed with Agilent TC-C18.A mixture of acetonitrile-0.2% trifluoroacetic acid-water（40∶20∶40）was used as the mobile phase with the flow rate at 1.0 mL·min-1.The detection wavelength was set at 254 nm.The Saikosaponin-b2 was extracted by ethyl acetate from plasma at the alkaline condition.Results Excellent liner relationship was obtained from the range of 0.05 mg·L-1 to 6.00 mg·L-1（r=0.999 7）.The lower limit of quantitation is 0.05 mg·L-1.The intra-day RSD were 5.20%,4.80%and 2.84% and inter-day RSD were 6.39%,5.13% and 2.47% respectively at three concentrations（0.1,1.0,4.0 mg·L-1）,and the relative recoveries were（97.17±7.22）%,（103.87±4.28）% and（101.75±1.54）% respectively.Conclusion The method was simple,rapid,accurate for determining the saikosaponin b2 concentration in rat plasma and for pharmacokinetics study.