中文摘要：

目的：建立柴胡汤剂中酰化柴胡皂苷a、b2的含量测定方法。方法：采用单因素分析和正交实验设计法，选择pH、超声时间、超声功率为因素各取3水平，按Lq（3^4）正表进行实验，优化汤剂中酰化柴胡皂苷a、b，的水解方法。采用HPLC法测定柴胡皂苷的含量。色谱条件：采用WondaSilC18色谱柱（4．6mm×250mm，5μm），以甲醇-水（53：47）为流动相，流速1．0mL·min^-1，检测波长210nm（检测酰化柴胡皂苷a）、250nm（检测酰化柴胡皂苷b2）。结果：优化的酰化柴胡皂苷水解方法为pH为10．0，超声（功率为500W，频率40kHz）40min；酰化柴胡皂苷a含量占汤剂中柴胡皂苷a含量的29％-41％，而酰化柴胡皂苷b，的含量占汤剂中柴胡皂苷b2的90％～115％。结论：该方法简便可靠，可用于汤剂中酰化柴胡皂苷a、b，的含量测定，并可为完善柴胡汤剂质量评价提供参考。

英文摘要：

Objective： To establish the determination method of acyl-saikosaponins a and b2 in Radix Bupleufi decoction. Methods： Single factor analysis and orthogonal experimental design were used to optimize the acyl- saikosaponin hydrolysis parameters such as pH, time of ultrasound-assisted hydrolysis and ultrasound power by L9 （ 3^4 ） orthogonal test. An HPLC method was employed to determine the saikosaponins contents, which was carried out on a WondaSil C18 column （ 4.6 mm × 250 mm, 5 μm ） with the mobile phase of methanol-water （ 53 ： 47 ） with the flow rate at 1.0 mL· min^-1 and the wavelengths of 210 nm for acyl-saikosaponin a and 250 nm for acyl-saikosaponin by Results： The optimized parameters of acyl-saikosaponin were listed as follow： the pH value was 10.0, the time of ultrasound-assisted hydrolysis was 40 min with ultrasound power of 500 W and ultrasound frequency of 40 kHz. In Radix Bupleuri decoction, the content of acyl-saikosaponin a accounted for 29%-41% of saikosqaponin a and acyl-saikosaponin b2 accounted for 90%-115% of saikosaponin b2. Conclusion： This method is simple and reliable, and can be used for the determination of acyl-saikosaponins a and b2 in Radix Bupleuri decoction and gives clues for the better quality assessment of Radix Bupleuri decoction.