中文摘要：

目的考察柴胡皂苷a、d（中药柴胡有效成分,为一对差向异构体,结构相近）在柴胡煎煮过程中的溶出和转化行为。方法采用HPLC测定柴胡煎煮过程中煎液和药渣在不同时间点各柴胡皂苷的含量变化。色谱条件为WondaSil C18色谱柱（4.6 mm×250 mm,5μm）;流动相：甲醇-水（53∶47）;流速：1.0 mL·min-1;检测波长：210,254 nm。结果柴胡皂苷a、d在柴胡水煎剂中的溶出和转化行为差异较大。主要体现在：1柴胡水煎液中可以检测到柴胡皂苷a,但检测不到柴胡皂苷d,药渣中柴胡皂苷a和d均能检测到;2无论在煎液还是在药渣中,柴胡皂苷a均少部分降解为柴胡皂苷b1,而柴胡皂苷d在水煎剂中全部转化为柴胡皂苷b2,在药渣中部分转化为柴胡皂苷b2。结论柴胡水煎液中不存在柴胡皂苷d,取而代之的是其转化产物柴胡皂苷b2。本实验为从水煎剂的角度认识柴胡的有效成分提供参考。

英文摘要：

OBJECTIVE To study the dissolving behaviors of saikosaponin a and d, a pair of epimeride and the main effective substances in Radix Bupleuri, during the decoction. METHODS HPLC method was employed to determine the contents of saikosa- ponins in the saiko decoction and its dregs at different decocting time, which was carried out on a WondaSil Cls column （4. 6 mm · 250 mm, 5 txm） with the mobile phase of methanol-water （53： 47） at the flow rate of 1.0 mL ·min-1 , and the wavelengths were 210 and 254 nm. RESULTS During the decocting process saikosaponin a could be detected in the decoction, however, saikosaponin d could not, which only was found in the dregs of saiko. And saikosaponin a could partly transformed into saikosaponin b1, but saikosaponin d totally changed into saikosaponin b2 in the decoction. Furthermore, in the dreg far less saikosaponin a was transformed than saikosapo- nind. CONCLUSION In the saiko decoction no saikosaponin d could be detected, but its degradation compound saikosaponin b2 could be found in a large amount. This paper provides clues for the research on the effective substances in the decoction of Radix Bu- pleuri.