中文摘要：

目的：在前期体内实验（文章已经发表）证实上皮-间质细胞转化核转录因子twist、snail在肺岩宁方抗肿瘤转移具有重要作用的基础上,进一步体外实验观察肺岩宁方对人高转移95-D肺癌细胞侵袭能力及twist、snail及Sip1基因和蛋白表达的影响。方法：应用不同浓度肺岩宁含药血清干预治疗人高转移肺癌细胞95-D的基础上,采用Tran-swell小室法检测细胞侵袭能力,并应用real-time PCR及Western blot方法检测对上皮-间质细胞转化核转录因子twist、snail及Sip1基因和蛋白表达的影响。结果：Transwell上室加入不同浓度肺岩宁方含药血清后,15%、20%及25%的肺岩宁含药血清的穿膜数量明显低于对照组（P〈0.01）;Real-time PCR结果表明：与对照组相比较,不同浓度肺岩宁含药血清对Twist均有下调作用（P〈0.01）,5%、10%、15%浓度的含药血清及DDP对Snail有下调作用（P〈0.01）;10%、15%肺岩宁方含药血清及DDP对Sip1有下调作用（P〈0.05或P〈0.01）。Western blot结果表明：5%、10%肺岩宁方含药血清具有显著下调Twist蛋白表达的作用（P〈0.01）,5%、15%浓度的肺岩宁方含药血清对Snail有下调作用（P〈0.01）,15%肺岩宁方含药血清对Sip1蛋白有下调作用（P〈0.01）。结论：肺岩宁方通过抑制Twist、Snail及SIP1的表达,从而抑制肿瘤异质黏附能力和运动能力来参与肺癌的侵袭和转移。

英文摘要：

Objective：Early in vivo experiments（article published） confirmed epithelial-mesenchymal transition of nuclear transcription factor twist and snail had important effect on Feiyanning decoction for its anti-tumor metastasis role.This in vitro study is to further explore Feiyanning＇s influence on highly metastatic lung cancer 95-D cells migration and twist,snail and Sip1 gene and protein expression based on previous research.Methods：Feiyanning medicated serum of different concentrations was used to intervene human highly metastatic lung cancer 95-D cells,Transwell assay method was used to detect cell invasion,real-time PCR and Western blot method was applied to detect twist,snail and Sip1 gene and protein expression.Results：After upper chamber of transwell was added with different concentrations of Feiyanning medicated serum,transmembrane of 10%、15%、20%medicated serum was significantly lower than that in the control group（P0.01）.Real-time PCR results showed that compared with the control group,Twist expression was significantly decreased after treatment with different medicated serum（P0.01）,Snail expression was significantly decreased after treatment with5%、10%、15%medicated serum and DDP（P0.01）,Sip1 expression was significantly decreased after treatment with of 10%、15%medicated serum and DDP（P0.05或P0.01）;Western blot results showed that compared with the control group Twist expression was significantly decreased after treatment with5%、10%medicated serum;Snail expression was significantly decreased after treatment with 5%、15%medicated serum;Sip1expression was significantly decreased after treatment with 15%medicated serum.Conclusion：Anti-metastasis mechanism of Feiyanning was to inhibit the expression of Twist、Snail and Sip1,which inhibit the adhesion of tumor heterogeneity,and athletic ability to participate in cancer invasion and metastasis.