中文摘要：

为了揭示Eaf2对胃癌MGC-803细胞增殖、侵袭和迁移的影响,使用定量Real-time PCR分析了50例胃癌组织（C）及其癌旁（N）中Eaf2的表达。用pcDNA3.1载体构建过表达Eaf2质粒（pcDNA-Eaf2）,以空载体pcDNA3.1（pcDNA-NC）作为对照,利用CCK-8法检测细胞增殖,Transwell小室和划痕实验检测细胞侵袭能力和迁移能力。Real-time PCR结果显示42例（84%）胃癌组织Eaf2表达下调,提示Eaf2作为抑癌基因起作用。过表达Eaf2能显著抑制MGC-803细胞的增殖、迁移和侵袭能力（P〈0.05）。结果提示Eaf2表达下调可能与胃癌的发展进程有关。

英文摘要：

To investigate the effects of Eaf2 on cell proliferation,migration and invasion of gastric cancer cell lines MGC-803,the level of Eaf2 expression was detected by real-time RT-PCR in 50 paired gastric cancer tissues and their adjacent normal tissues.Eaf2 overexpression was achieved by transfection of construct pcDNA-Eaf2 into MGC-803 cells.The proliferation,migration and invasion of MGC-803 cells were detected by the CCK-8,scratch wound and transwell assay respectively.The real-time PCR results indicated that 42cases（84%）of gastric cancer tissues were decreased.Think that Eaf2 plays an important role in gastric cancer as a tumor suppressor,overexpression of Eaf2 can effectively inhibit the proliferation,invasion and migration of MGC-803cells（P〈0.05）.The results suggested that down-regulation of Eaf2 had a correlation with the progression of gastric cancer.