中文摘要：

为了探讨单细胞真核生物八肋游仆虫酸性核糖体蛋白P1（EoP1）的磷酸化作用,对EoP1的一个亚型EoP1A进行了磷酸化位点分析。通过在线软件预测EoP1A的磷酸化位点,根据预测结果进行定点突变,随后对EoP1A野生型及突变体分别在大肠杆菌中进行了表达纯化。利用核磁共振（NMR）检测EoP1A的体外磷酸化作用,结果表明位于蛋白N端的第8位丝氨酸（Ser8）为CK2磷酸化EoP1A蛋白的磷酸化位点。

英文摘要：

Towards understanding the phosphorylation function of the acidic ribosomal protein EoP1 from the unicellular eukaryotes Euplotes octocarinatus, the phosphorylation site of its subform EoP1A was ana- lyzed. Based on the prediction result of the peptide sequence analysis using the NetPhos 2.0 software pro- gram, two mutants EoP1A-SSA and EoPIA-AY were constructed using site-directed mutagenesis. Both mutants and wild-type （EoP1A-WT） were expressed in E. coli and purified. The NMR results showed that the EoP1A-WT and EoP1A-AY but not the EoP1A-S8A were phosphorylated in vitro by CK2, suggesting that EoP1A was phosphorylated by CK2 in vitro at Ser8 in the N-terminal domain.