中文摘要：

目的用紫外分光光度法和高效液相色谱法测定注射用灯盏花乙素中灯盏花乙素的含量。方法紫外分光光度法用甲醇作为溶剂,选择335nm作为测定波长。高效液相色谱法采用Luan C18（150×4.6 nm）作为分析柱,流动相为0.05%的磷酸水—乙腈梯度洗脱,柱温35℃,流速1 m L/min,检测波长335 nm。结果紫外分光光度法,灯盏花乙素在5.0～15.0μg/m L范围内与吸光度呈良好线性关系（r=0.999 85）,平均回收率为99.20%（n=9）,RSD=0.82%（n=9）,精密度与重复性RSD良好,分别为0.15%和0.93%;高效液相色谱法,灯盏花乙素在10.0～80.0μg/m L范围内与峰面积呈良好的线性关系,r=0.999 8,平均回收率为100.69%.结论紫外分光光度法虽然简便、快速,但专属性不好,易受外界环境影响。所以综合考虑选择用高效液相色谱法测定注射用灯盏花乙素含量比较优。

英文摘要：

Objective To determine the content of the injection Breviscapine by UV spectrophotometry and high performance liquid chromatography. Methods UV spectrophotometry using methanol as solvent,choose 335 nm as wavelength. High performance liquid chromatography using Luan C18（150 ×4.6nm） as analytical column and the mobile phase was 0.05% phosphate water-acetonitrile gradient Elution. The column temperature was 35 ℃,the flow rate was 1m L/min,the detection wavelength was set at 335 nm. Results UV spectrophotometry,Breviscapine in 5. 0～15. 0μg/m L within the range of absorbance was linear（r=0.99985）,the average recovery was 99.20%（n =9）,RSD =0.82%（n =9）,precision and repeatability RSD good,respectively for 0. 15% and 0.93%;HPLC Breviscapine in 10.0 ～80.0μg/m L range and peak area showed a good linear relationship,r =0.9998,the average recovery was 100.69%. Conclusion Although UV spectrophotometry is simple,fast,but specificity is poor,susceptible to external environmental impacts. Therefore,considering the selective determination injection comparative advantage Breviscapine content by HPLC.