中文摘要：

以感染水霉病的鲤（Cyprinus carpio）为研究对象,测定了患病鲤血浆中氧化应激相关指标,包括总抗氧化能力（T-AOC）、脂质过氧化产物-丙二醛（MDA）、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）,同时随机取患病组鲤的肝胰脏、脾脏和肾脏组织进行组织病理分析,旨在探讨氧化应激同水霉菌致病机理的关联。结果表明,1患病组相对于对照组,鱼体血浆T-AOC极显著降低（P〈0.01）;MDA含量和GSH含量无显著变化;SOD和CAT活性极显著降低（P〈0.01）;GPx活性显著升高（P〈0.05）,表明鲤患水霉病后鱼体内氧化水平-抗氧化水平失衡,鱼体产生氧化应激。2组织病理学分析显示患水霉病鲤的肝脏细胞相对于对照组出现严重的急性坏死症状,细胞出现空泡化现象严重;脾脏和肾脏细胞出现不同程度的炎症,细胞开始凋亡。

英文摘要：

In this study, plasma from diseased or healthy common carps （Cyprinus carpio） was collected to evaluate the effect of infection with Saprolegniasis on oxidative stress. Several bio-chemical markers of oxidative stress, including the total antioxidant capacity （T-AOC）,lipid peroxidationmalondialdehyde （MDA）,superoxide dismutase （SOD）, glutathione （GSH）, glutathione peroxidase （GPx）,and catalase （CAT） were measured among controls and infected common carps. Compared with the healthy common carps,some biomarkers of the common carps infected with Saprolegniasis, including T-AOC,SOD and CAT,were decreased significantly （P〈0.01）,and the plasma GPx was increased significantly （P〈0.05）. Other biomarkers of plasma MDA and GSH showed no significant change after the infection with Saprolegniasis. These results indicated that oxidative stress has occurred upon Saprolegniasis infection. The histopathology damage of liver, spleen, and kidney tissues from diseased fish were also observed after hematoxylin and eosin （HE）staining. Liver cells of common carps infected with Sapro- legniasis was presented with the symptom of acute necrosis, and became vacuolization seriously;spleen and kidney cells was presented with inflammations of different severity,senescence and apoptosis.