中文摘要：

目的建立高效液相色谱测定5,6,7,4’-四乙酰氧基黄酮含量的方法。方法色谱柱Luna C18（4.6mm×150mm,5μm）,乙腈-0.02%磷酸（50∶50）为流动相,流速1m L/min,检测波长254nm,柱温35℃。结果经方法学验证表明,精密度、稳定性、重现性、线性范围、回收率等均符合要求。结论该方法简便、快速、准确,可用于5,6,7,4’-四乙酰氧基黄酮的含量测定和质量控制。

英文摘要：

Objective Establishing a HPLC method for the determination of scutellarein tetraacetate. Methods Column：Luna C18 （4.6mm×150mm, 5μ）; Aeetonitrile-0.02% phosphoric acid （50：50） was used as the mobile phase at a flow rate of 1 mL/min, the detection wavelength was 254 nm, column temperature was 35℃. Results Methodology validation showed that the precision, stability,repeatability,linear range and average recovery rate all conforms to the requirement. Conclusion The method was so convenient, fast and accurate that it can be used in the assay and the quality control of scutellarein tetraacetate.