中文摘要：

目的：研究纳米ZnO颗粒对小鼠肺泡巨噬细胞吞噬能力和炎性因子的影响。方法：采用不同尺寸的纳米ZnO颗粒对小鼠肺泡巨噬细胞进行染毒,通过中性红法测定小鼠肺泡巨噬细胞的吞噬能力,采用酶联免疫法测定炎性因子。结果：高浓度时（20μg/ml）,小鼠肺泡巨噬细胞的吞噬能力明显降低;浓度为5～20μg/ml时,TNF-α、IL-6和IL-8均显著升高,并且10～30 nm ZnO颗粒处理组TNF-α和IL-6升高显著高于其他ZnO颗粒处理组。结论：纳米ZnO颗粒降低小鼠肺泡巨噬细胞的吞噬能力并引起炎性反应。

英文摘要：

Objective： To study the effect of ZnO nanoparticles on phagocytic function and inflammatory reaction of mouse alveolar macrophages.Methods： After exposure of Mouse alveolar macrophages to different sized ZnO nanoparticles,phagocytic function was determined by the neutral red method,inflammatory factors were determined by Enzyme-linked immunosorbent assay.Results： Phagocytic function of mouse alveolar macrophages was significantly reduced at high dose（20 μg/ml）.At the dose of 5～20 μg/ml,the contents of TNF-α,IL-6 and IL-8 were marked increased.10～30 nm ZnO particles induced higher levels of TNF-α and IL-6 thanother three ZnO nanoparticles.Conclusions： ZnO nanoparticles reduced phagocytic function and caused inflammation.