中文摘要：

目的：探讨减少脑组织冰冻切片脱片和增加脑组织冰冻切片免疫组织化学染色结果准确性的方法,为脑组织冰冻切片的广泛使用进一步奠定实验基础。方法：在保证实验真实性和科学性的前提下,将现有的脑组织冰冻切片技术结合在脑组织冰冻切片过程中所遇到的问题,以明胶硫酸铬钾处理载玻片,预冷的NS和4%多聚甲醛灌注固定组织,液氮速冻脑组织进行冰冻切片。结果：明胶硫酸铬钾处理载玻片的方法减少了冰冻切片脱片,预冷的NS和4%多聚甲醛提高了脑组织灌流固定效果,液氮速冻脑组织减少了冰晶产生。结论：此方法使脑组织冰冻切片脱片情况下降,冰晶产生减少,提高了脑组织冰冻切片免疫组织化学染色结果的准确性,该方法值得在临床和科研实验室中积极推广使用。

英文摘要：

Objective：To probe a practical and convenient approach of reducing the detachment of brain tissue sections from glass slides and improving the of experiments.Methods：The new methods was studied in the progresses of treating glass slides,brain tissue perfusion fixation,frozen section and immunohistochemistry.Results：Treating of chromic potassium sulfate on glass slides reduced the detachment of brain tissue section;cold NS and 4% paraformaldehyde improved tissue perfusion fixation;the method,that liquid nitrogen frozen brain tissue quickly,reduced ice crystal.Conclusion：The detachment of brain sections can be reduced;immunohistochemical results of brain frozen section can be improved,the method is worth spreading among clinical and scientific researches.