中文摘要：

目的 探讨缺血性脑卒中大鼠缺血周边脑组织Caveolin-1（Cav-1）的表达及抑制其表达对缺血周边血管新生的影响。方法 随机将雄性Wistar大鼠分为4组：假手术组（Sham组,n=15）,脑缺血组（MCAO组,n=55）,Cav-1 siRNA慢病毒转染组（siRNA组,n=20）以及非编码siRNA慢病毒转染组（misRNA组,n=20）。用Q-PCR以及免疫印迹检测Cav-1的动态表达,FITC-dextran检测血管密度,多普勒血流仪检测血流量。结果 与Sham组比较,MCAO组再灌注4 h~3 d,Cav-1表达逐渐下降（P均〈0.05）,3 d表达水平最低（P〈0.01）,7 d后明显恢复（P〈0.05）,14 d接近正常（P〉0.05）。与MCAO组比较,siRNA组Cav-1的表达水平显著降低,并且缺血周边血管密度及血流量显著下降（P均〈0.05）;misRNA组的上述指标无明显变化。结论 Cav-1参与调节缺血性脑卒中后缺血周边脑组织的血管新生。

英文摘要：

Objective To investigate the expression and involvement of Caveolin-1（ Cav-1） in postischemic angiogenesis using a rat model of ischemic stroke. Methods Normal male Wistar rats were randomly divided into four groups：Sham group（ n = 15）,cerebral ischemia group（ MCAO group,n = 55）,lentivirus-expressing Cav-1 siRNA treated group（ siRNA group,n = 20） and lentivirus-expressing nonsense siRNA treated group（ misRNA group,n = 20）.Q-PCR and Western blotting analysis were used to detect the expression of Cav-1. FITC-dextran was used to detect the vessel volume,and Laser Doppler was used to measure the local cerebral blood flowin ischemic penumbral. Results The expressions of Cav-1 from hour 4 to day 3 post-MCAO significantly decreased in MCAO group（ all P〈0. 05）,compared with sham group,and the minimum level appeared on day 3 post-MCAO（ P〈0. 01）. Interestingly,the expression of Cav-1 on day 7 post-MCAO significantly restored（ P〈0. 05）,and reached the normal level on day 14post-MCAO（ P〉0. 05）. As expected,in Cav-1 siRNA group,the expression of Cav-1 significantly decreased compared with MCAO or misRNA group（ all P〈0. 05）. Meanwhile,there were significantly weakened vessel volume and local cerebral blood flowin ischemic penumbral（ P〈0. 05）. Conclusion Cav-1 might be involved in postischemic angiogenesis. Further investigation of the regulation molecular machenism of Cav-1 in postischemic angiogenesis may be helpful for therapeutic angiogenesis for ischemic stroke.