中文摘要：

提出了一种利用RNA核酸识体和光开关化合物[Ru（phen）2dppz]^2＋（RU）高灵敏检测药物分子的荧光分析新方法．以托普霉素（TOB）为靶分子，检测限可达到10nmol／L，同时该方法有较好的选择性，能在溶液中实现无需分离的实时检测．这种无标记检测方法有利于不稳定RNA核酸识体在分析检测中的应用，简化实验操作。为其它药物分子的检测提供了新思路．

英文摘要：

Aptamers are a new class of oligonucleotides generated from in vitro selection with a high affinity and specificity. In this paper, a novel luminescence detection method was developed by using RNA aptamers and a DNA light switching complex, [ Ru （phen）2 （dppz） ] ^2＋, as a signaling aptamer for the detection of drug tobramycin. The results showed that the method was highly sensitive and selective with the detection limit down to 10 nmol/L. The method was also very simple and could realize direct detection without separation in solution, and offers a new approach for the exploitation and application of aptamers in drug analysis.