中文摘要：

人线粒体转录终止因子4（mitochondrial transcription termination factor 4,MTERF4）基因属于线粒体转录终止因子基因超家族,其编码的蛋白质是调控线粒体核糖体生物合成和线粒体蛋白质翻译的重要因子。目前,MTERF4基因自身的表达调控机制仍不清楚。为了探讨人MTERF4基因启动子区的序列特征和基因转录调控机制,采用进化足迹法,利用生物信息学在线软件对人MTERF4基因上游5＇端的启动子分布、转录因子及其结合位点、CpG岛进行分析。结果表明,人MTERF4基因上游5＇端3 000 bp的核苷酸序列至少存在3个启动子,其中1 799-2 368 bp之间可能包含核心启动子。启动子区序列中存在1个长为558 bp的CpG岛。人和小鼠MTERF4同源基因的启动子区有1个保守区域,存在18个共有的转录因子结合位点。通过生物信息学软件分析人MTERF4基因启动子,提高了针对该基因启动子的研究效率,为后续实验深入研究启动子的功能奠定了重要的理论基础。

英文摘要：

Human mitochondrial transcription termination factor 4（MTERF4） gene belongs to MTERF gene superfamily.MTERF4 protein plays an important role in the mitochondrial ribosome biogenesis and the regulation of mitochondrial translation.However,the potential regulation mechanism of human MTERF4 gene expression is still undiscovered.In order to investigate the regulation mechanism of human MTERF4 gene promoter,online bioinformatics software and phylogenetic foot-printing methods were used to analyze the promoter distribution,transcription factors and their binding sites,CpG islands of human MTERF4 gene.The results showed that there are at least three promoters in the 5′ uncoding region of human MTERF4 gene.The core promoter of human MTERF4 gene is located between 1 799 bp and 2 368 bp,which plays a key role in the transcription of MTERF4.In addition,18 transcription factor binding sites were found in conserved promoter region of human and mouse homologous MTERF4 genes by P-Match 1.0 protocol.A 558 bp CpG island was found in human MTERF4 gene promoter region.Bioinformatics analysis of human MTERF4 gene promoter can improve the efficiency of promoter research,and provide significant information for the further experimental research of gene promoter function.