中文摘要：

目的：探讨人线粒体转录终止因子3基因启动子区的序列特征、转录因子及其结合位点。方法：利用Promoter2.0、NNPP、Proscan、FirstEF软件分别预测hMTERF3基因5端上游的启动子数目及分布；利用CpGIslandSearcher和CpGPlot软件预测CpG岛位置；利用P-match1.0程序搜索TRANSFAC数据库预测与hMTERF3基因启动子结合的转录因子及其结合位点。结果：hMTERF3基因定位于8q21.2，基因全长22216bp，含有11个外显子和10个内含子。hMTERF3基因上游至5'侧翼共3000bp的核苷酸序列至少存在2个启动子区，其中1733~2302bp之间可能为包含TATA盒的核心启动子区。启动子区序列中存在1个长为1145bp的CpG岛。hMTERF3基因启动子区存在1055个转录因子结合位点，进化足迹法分析其保守的核心启动子区转录因子结合位点共19个。结论：hMTERF3基因启动子区的生物信息学分析能够提高基因启动子的研究效率，为后续实验构建hMTERF3基因启动子表达载体及鉴定启动子功能提供理论依据。

英文摘要：

Objective: To investigate the characters, transcription factors and their binding sites of human mitochondrial transcription termination factor 3(hMTERF3)gene promoter by different bioinformatics tools. Methods: Promoter 2.0, NNPP, Proscan, and FirstEF softwares were used to analyze the numbers and distributions of hMTERF3 gene promoter; CpG Islander and CpG Plot softwares were used to analyze the GpG island of hMTERF3 gene promoter; P-match 1.0 protocol and TRANSFAC database were used to analyze the transcription factors and their binding sites of hMTERF3 gene promoter. Results: hMTERF3 gene was located on 8q21.2. The full length of hMTERF3 gene was 22 216 bp, consisting of 11 exons and 10 introns. There were at least two promoters in the 5' unconding region of hMTERF3 gene. The core promoter of hMTERF3 gene was located between 1 733-2 302 bp, containing TATA box. In hMTERF3 gene promoter region, a 1 145 bp CpG island could be found. In addition, 1 055 transcription factor binding sites were predicted by P-Match 1.0 protocol, and only 19 transcription factor binding sites were found in conserved core promoter region of human and mouse homologous MTERF3 genes by phylogenetic foot- printing analysis. Conclusion: Gene promoter related bioinformatics analysis can improve the efficiency of hMTERF3 gene promoter research, and provide significant information for the construction of promoter expression vector, also for the further study of promoter function.