利用70对扩增产物具有稳定多态性的SSR标记研究了66份玉米自交系的遗传多态性。70对引物在供试材料中共检测出273个等位基因变异,每对引物检测等位基因2—7个,平均3.9个,每个位点的多态性信息量(HC)变化于0.121—0.814之间,平均0.584。66份自交系之间的遗传相似系数变化范围0.70-0.93。UPGMA聚类分析结果表明,66份供试自交系划分为5个类群,分类结果与系谱来源基本一致,东北地区生产上主要推广杂交种的亲本大多来自不同的类群。
Simple sequence repeats (SSRs) were used to detect genetic diversity among 66 maize (Zea mays L. ) inbred lines, which were widely used in northeast provinces of China. Seventy selected SSR primers gave stable profiles amplified in samples of 66 inbred lines. Seventy primers produced 273 polymorphic amplified fragments. The average number of allele per SSR locus was 3.9 which varied from 2 to 7. The polymorphism information content (PIC) for the SSR loci varied from 0. 121 to 0. 814 with an average of 0. 584. Genetic similarities among the 66 inbred lines ranged from 0.62 to 0.93. The cluster analysis showed that the inbred lines could be classified into 5 distinct clusters. The results were consistent with the groups based on the available pedigree. Most of the parents used in maize commercial hybrids came from opposite clusters.