中文摘要：

在玉米分子标记辅助育种工作中,需要对大量群体的个体样本进行分子标记分析,其中DNA的高效快速提取是关键的技术环节.以异硫氰酸胍为主要提取试剂,结合使用组织研磨器（Qiagen Tissuelyser）,建立了一种玉米叶片基因组DNA的快速提取新方法.利用此方法提取玉米叶片基因组DNA,具有使用药品少、操作步骤简单、快速、成本低、DNA质量稳定等优点,通常情况下每人每个工作日可以提取300个DNA样品.此DNA提取方法可有效地用于玉米分子标记分析.

英文摘要：

A rapid and simple DNA extraction is essential in molecular maker aided selection in maize as a large amount of samples needed for analysis. In this paper, lished with guanidiniumthiocyanate （GuSCN）, an indis a new method of genomic DNA extraction from maize was estabpensable reagent, combined with use of the Qiagen Tissuelyser. This method is less-reagent used, rapid and simple to run, cost-saving, etc. Three hundred DNA samples could be extracted each workday by per capita using this method, revealing that this method can be effectively used in marker analysis of maize.