中文摘要：

目的：探索CT灌注成像在兔肝脏缺血再灌注损伤（I/R）模型中的应用。方法：新西兰大白兔随机分成4组（正常对照组及I/R 6h、12h、24h组,每组5只）。I/R模型采用无创性动脉夹结扎肝左叶血供60min后,恢复血供。各组分别采用iCT行全肝灌注成像;在灌注图上测量肝动脉灌注量（HAP）、门静脉灌注量（HPP）、总灌注量（TLP）及肝动脉灌注指数（HPI）;同时行组织病理学检查。结果：与正常组对比,24h组HAP显著下降（P〈0.01）;各I/R组中HPP、TLP明显低于正常组（P〈0.05）;而在I/R 6h、12h组中,HPI显著高于对照组（P〈0.05）。镜下表现肝窦红细胞淤积,肝细胞核固缩凋亡,肝窦解离,在24h组出现局灶性坏死。结论：CT灌注成像能够动态准确反应肝I/R后微循环灌注量的变化情况。

英文摘要：

Objective：To prospectively investigate the value of computed tomography（CT） perfusion imaging for assessment of perfusion changes in a rabbit hepatic ischemia reperfusion injury（I/R） model.Methods：After induction of hepatic I/R by left liver lobe ischemia（60 min） followed by 6 h,12 h and 24 h reperfusion,a total of 20 rabbits（one control group and 3 I/R groups;n=5,for each） were imaged with a dynamic CT protocol.The perfusion indices of hepatic arterial perfusion（HAP）,hepatic portal perfusion（HPP）,total liver perfusion（TLP） and hepatic perfusion index（HPI） were measured after obtaining perfusion index maps.Liver samples underwent histological examination after every group had completed scan.Results：HAP in I/R 24 h group was significantly lower than that of the control group（P〈0.01）.The HPP and TLP of I/R groups were always lower than control group（P〈0.01）.In addition,the HPI in I/R 6 h and 12 h groups were significantly higher than the control group（P〈0.05）.Histopathology revealed the presence of erythrocytes deposited in hepatic sinusoids,followed by in ammatory cell infiltrating,hepatocyte nuclear condensation of apoptosis,and sinusoidal dissociation.With aggravated injury,patchy focal necrosis scattered in 24 h group.Conclusion：These results demonstrate the feasibility of CT perfusion parameters to assess the hemodynamic changes during the pathological process of rabbit hepatic I/R.