中文摘要：

这研究的目的是在 HCC 房间线 HepG2 观察指向的治疗的爱到原生质标志 AF-pGL3-hTERT-TK 的目的。我们构造了治疗学的原生质标志 pGL3-hTERT-TK (由 hTERT 的倡导者支持了的包含的自杀基因 TK ) 的方法并且与 AF-liposome (能在 HCC 房间表面上与受体 ASPGR 结合的蛋白质) 被结合。然后我们有 AF-pGL3-hTERT-TK 的 transfected HCC 房间线 HepG2 和肝的房间 L02，由流动 cytometry 和隧道方法在 vitro 为 HCC 房间线生长和 apoptosis 观察了治疗学的原生质标志 AF-pGL3-hTERT-TK 的效果。我们的结果显示出的结果那 TK 基因是在原生质标志 pGL3-hTERT-TK 的 1100 bp。有效地， transfect HCC 房间 HepG2 和 transfection 评估的 Plasmid pGL3-hTERT-TK 罐头是 8.91% 。由在 HCC 房间表面上认出并且联合受体蛋白质 ASPGR 的效果，治疗学的原生质标志 AF-pGL3-hTERT-TK 能容易进入 HCC 房间 HepG2 并且导致它的 apoptosis。apoptosis 率是 85.87% 当时仅仅 8.65% 在 L02 房间。四天在 AF-pGL3-hTERT-TK transfected HepG2 是由 ganciclovir (GCV ) 的干预以后，很多 apoptotic 身体被隧道分析发现，当小 apoptotic 身体在肝的房间 L02 被发现时。结论 AF-pGL3-hTERT-TK 能指向到 HCC 房间线并且导致它到 apoptosis，几乎没在肝的房间 L02 上有影响。AF-pGL3-hTERT-TK 为 HCC 有潜在的治疗学的效果。

英文摘要：

Objective： The aim of this study was to observe the affection of targeted therapy to plasmid AF-pGL3-hTERT-TK in HCC cell line HepG2. Methods： We constructed therapeutic plasmid pGL3-hTERT-TK （containing suicide gene TK that promoted by promoter of hTERT） and was conjugated with AF-liposome （a protein that can combine with the receptor ASPGR on HCC cell surface）. Then we transfected HCC cell line HepG2 and hepatic cell L02 with AF-pGL3-hTERT-TK, observed the effects of therapeutic plasmid AF-pGL3-hTERT-TK for HCC cell line growth and apoptosis in vitro by Flow cytometry and Tun- nel method. Results： Our results showed that TK gene was 1100 bp in plasmid pGL3-hTERT-TK. Plasmid pGL3-hTERT-TK can effectively transfect HCC cell HepG2 and the transfection rate was 8.91%. By recognizing and combining effects of recep- tor protein ASPGR on HCC cell surface the therapeutic plasmid AF-pGL3-hTERT-TK could easily enter into HCC cell HepG2 and induce its apeptosis. The apoptosis rate was 85.87% while only 8.65% in L02 cell. Four days after AF-pGL3-hTERT-TK transfected HepG2 was intervention by ganciclovir （GCV）, a lot of apeptotic bodies were found by Tunnel analysis, while little apoptotic body was found in hepatic cell L02. Conclusion： AF-pGL3-hTERT-TK can target to HCC cell line and induce it to apoptesis, almost has no influence on hepatic cell L02. AF-pGL3-hTERT-TK has the potential therapeutic effects for HCC.