中文摘要：

通过圆二色谱、稳态吸收光谱、稳态荧光光谱和皮秒时间分辩荧光光谱研究了meso四（4-（N-甲基吡啶基））卟啉（TMPyP4）与端粒DNA5’-TTAGGG-3’（S6）的结合机理．稳态光谱实验结果表明，在没有金属离子的Tris—HCI缓冲溶液中，s6DNA以单链的形式存在，TMPyP4与单链DNA的结合常数为1．51×10^6（mol／L）^-1，饱和结合数为1．2．通过时间分辨荧光光谱对二者相互作用的机理进行了进一步研究，推测了TMPyP4与单链S6DNA之间的结合模式．

英文摘要：

The binding mechanism of meso-tetrakis （4-（N-methylpyridiumyl））porphyrin （TMPyP4） and the telomeric sequence DNA 5＇-TTAGGG-3r （S6） has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 10^6 （mol/L）^-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.