中文摘要：

采用酶联免疫吸附检测（ELISA）试剂盒定量分析了某采用生物脱氮活性污泥工艺的污水厂中的原水、一级处理出水、二级处理出水、尾水和出水口下游受纳河水中的雌酮浓度，分析方法的定量检测限为0．5ng／L。ELISA分析中增加的凝胶色谱净化步骤可降低E1检测值73．0%的假阳性，使其检测结果平均值从液相色谱一飞行时间质谱联用（HPLC—MS（TOF））的对照分析结果平均值的1．52倍降为1．15倍。ELISA分析检测值的假阳性与水样的污染程度呈正相关。

英文摘要：

An estrone（E1）enzyme-linked immunosorbent assay（ELISA） kit is used for the quantification analysis of E1 concentrations in sewage and river water samples. Raw water, primary treatment e/fluent, secondary effluent, final effluent and downstream river water samples are collected from a sewage treatment works using the nitrifying activated sludge process. The analysis detection limit is 0. 5 ng/L. The average absolute concentrations measured by ELISA are 1. 52 times higher than those measured by parallel liquid chromatrography coupled with time-of-flight mass spectrometry（HPLC-MS（TOF）） without gel chromatography. These ELISA results are reduced to 1.15 times the HPLC-MS（TOF） results with gel chromatography cleanup. False positives of ELISA analysis are decreased by 73.0% and are positively correlated with the sewage strength.