中文摘要：

目的探讨长期冻存后的大胚龄人胚神经干细胞（neural stem cells,NSCs）的生长及其生物学特性。方法分别于冻存后6、12及24个月,快速解冻复苏胚龄为16～20周的胎脑细胞,采用无血清培养液悬浮培养,观察培养细胞的活性及成球时间,采用免疫细胞化学方法鉴定这些细胞球及其分化成熟后的细胞表面标志。结果培养传代的细胞透亮﹑成球时间短,与取自新鲜胎脑的神经干细胞无明显区别。细胞球Nestin染色阳性,成熟分化后神经元特异性烯醇化酶（NSE）、胶质纤维酸性蛋白（GFAP）及2＇,3＇-环腺苷酸-3＇-磷酸二酯酶（CNPase）抗原呈阳性。结论长期冻存后的人大胚龄神经干细胞仍具有较强增殖能力及多分化潜能。

英文摘要：

Objective To study the growth and property of thawing old truman embryonic neural stem cells （NSCS） after long cryopreservation. Methods After 6,12 and 24 months, the cells isolated from about 16-20 months fetus were thawed and cultured in serum-free medium. The cells were observed and identified with inverted microscopy and immunocytochemistry. Results The cells grew very well after thawing, and presented an ability of consecutive cloning and differentiation. The clusters of cells were positive by Nestin immunocytochemical staining, NSE, GFAP and CNPase antigens were positive in the mature cells. Conclusion The NSCS harvested from old human fetus in vitro have the ability of proliferation and potential of multi-directional differentiation after long cryopreservation.