中文摘要：

目的初步探讨稀土化合物氯化镧（Lanthanum chloride，LaCl3）对体外培养的NSCs增殖状况的影响。方法采用成球悬浮法分离培养小鼠神经干细胞（mNSCs）；将生长状态良好的第3代mNSCs分为空白对照组、肿瘤坏死因子-α（TNF-α）组、LaCl3组，分别采用常规NSCs培养液、含10ng／ml TNF-α的NSCs培养液、含2.5μmol／L LaCl3的NSCs培养液培养NSCs 1-3天。采用神经球计数、神经球体积测量方法以及采用免疫荧光细胞化学方法检测与细胞增殖相关的指标（CyclinD1）来观察NSCs的增殖状况。结果神经球计数和神经球体积测量结果显示，TNF-α组和LaCl3组神经球数量分别为[（74.56±2.6）个／瓶]和[（62.32±2.8）个／瓶]，较空白对照组[（42.28±3.5）个／瓶]明显增多，P〈0.05；神经球体积分别为[（0.82±0.16）×10^6（μm^3）]和[（0.66±0.12）×10^6（μm^3）]，较对照组[（0.26±0.0.08）×10^6（μm^3）]明显增大，P〈0.05。免疫细胞荧光检测细胞增殖结果显示，TNF-α组和LaCl3组中，Cyclin D1阳性细胞率分别为（68.6±4.2）％和（51.2±2.8）％，明显高于空白对照组的（35.6±2.6）％，P〈0.01。结论一定浓度的Lacl3具有促进NSCs增殖的作用。

英文摘要：

objective To investigate the effects of Lanthanum chloride（LaCl3） on proliferation of neural stem cells （NSCs）. Methods The mouse NSCs（mNSCs） were isolated and divided randomly into 3 groups： control group, TNF-α group and LaCl3 group. In control group, mNSCs were cultured in NSCs media routinely; In TNF-α group, the cells were cultured in NSCs media containing 10ng/ml TNF-α; mNSCs in LaCl3 group was treated with NSCs media containing 2.5μmol/L LaCl3. The mNSCs in each group were cultured for 1-3 days. The total number of neurospheres was counted and the average volume of neurospheres was measured respectively. Immunofluorescence staining was employed to detect CyclinD1 involved proliferation of NSCs. Results The total number of neurospheres in TNF-α and LaCl3 groups were 74.56±2.6 and 62.32±2.8 per flask respectively, which was more than that of control groups 42.28±3.5 per flask significantly, P〈0.05 The volumes of neurospheres in TNF-α and LaCl3 groups were 0.82±0.16×10^6μm^3 and 0.66±0.12×10^6μm^3 respectively, which was bigger than that of control group （0.26±0.08）×10^6μm^3 significantly, P〈0.05. The results of immunofluorescence staining showed the positive rates of CyclinD1 in TNF-α and LaCl3 groups were 68.6±4.2% and 51.2±2.8% respectively, both were significantly higher than that of control group （35.6±2.6）%, P〈0.01. Conclusion A certain dosage of Lanthanum chloride is able to promote NSCs proliferation.