中文摘要：

采用RT-PCR、RACE技术从盐生植物盐节木（Halocnermum strobilaceum（Pall．）Bieb．）中克隆得到BADH基因的eDNA序列（命名为HsBADH），3’RACE和5’RACE测序结果用DNAMan软件拼接获得了1849bp全长的HsBADHeDNA序列（GenBank登录号：JN969894）。其开放阅读框为1503bp，推测编码501个氨基酸残基，并含有高度保守的十肽基序（VTLELGGKSP）和与酶功能有关的醛脱氢酶Cys残基。其核苷酸序列与几种藜科植物如梭梭、白梭梭、盐穗木、碱蓬、滨藜、菠菜、山菠菜的同源性为86％，与甜土植物水稻的同源性为66％。氨基酸序列与以上两类植物（盐生植物和甜土植物）的同源性比对分别为89％和70％，说明BADH基因在藜科盐生植物中是一种较高保守的基因。用不同浓度的NaCI胁迫处理盐节木植株，BADHmRNA的表达水平比对照植株高，说明盐节木BADH基因的表达受盐诱导，进一步说明甜菜碱醛脱氢酶催化合成的甜菜碱作为渗透调节的小分子物质，它的积累与盐胁迫存在紧密关联，本研究为进一步从生理和分子水平阐明盐节木的适应盐渍化生境的机制提供一定的参考。

英文摘要：

According to the published sequences of BADH cDNA of several other plants of Chenopodiaceae, two primers have been designed to amplify the fragments of BADH cDNA from Halocnermum strobilaceum （Pall.） Bieb. through RT-PCR （reverse transcription polymerase chain reaction）. A 1 503 bp fragment containing entire betaine .aldehyde dehydrogenase （BADH）coding region of 501 amino acids （aa） has been obtained. Nucleotide sequence of HsBADH was similar to the corresponding fragment of BADH cDNA of several other plants, such as Kalidium foliatum, Atriplbx centralasiatica, Atriplex hortensis, Spinacia oleracea, Suaeda liaotungensis, Beta vulgaris subsp. Vulgaris, Oryza sativa, etc. Encoded protein by HsBADH and BADH protein from above mentioned plants also shared 89% and 70% identity at the amino acid level. The result showed that BADH gene was conserved, especially in Chenopodiaceae and encoded functional protein may play an important role in high plants during salt stress. Real-time fluorescence quantitative gene expression analysis showed that the level of BADH mRNA in plants treated with different NaC1 concentrations was higher than that in the control plants, suggesting that the accumulation of betaine catalyzed by betaine aide-hyde dehydrogenase as an effective osmolyte is important for H. strobilaceum （ Pall. ） Bieb. during salt stress. The study provided material for further exploring salt tolerant mechanisms of H. strobilaceum（ Pall. ） Bieb. in physiological and molecular aspects.