中文摘要：

目的从生物物理与生物化学的角度，探讨黄连和吴茱萸“配伍-煎煮”顺序对左金丸和反左金药性的影响。方法采用微量热法，测定大肠埃希菌在黄连和吴茱萸“配伍-合煎”组及“分煎-配伍”组的左金丸和反左金水煎液作用下的生长热谱曲线，获得相应的生物热动力学参数。根据热动力学模型，拟合药物的k～c和I％～c关系，定量计算药物的半抑制浓度（IC50）。结合本草文献报道，分析“配伍-煎煮”顺序对左金丸和反左金药性的影响。结果黄连和吴茱萸“配伍-煎煮”顺序对左金丸和反左金药性有一定影响。反左金对细菌生长有促进作用，而左金药液均能抑制细菌的生长，“分煎-配伍”组左金丸水煎液的IC50（5．63mg·mL^-1）明显小于“配伍-合煎”组左金丸水煎液的圮。（7．40mg·mL^-1）表明“分煎-配伍”组的药物对大肠埃希菌的抑制作用强于“配伍-合煎”组。结论微量热法可用于考察黄连和吴莱萸“配伍-煎煮”顺序对左金丸和反左金药性的影响。

英文摘要：

Objective To investigate the influence of the sequence of ＂ compatibility-decoction＂ for rhizoma coptidis and fructus evodiae on medical property of zuojinwan and fanzuofin based on biophysics and biochemistry. Methods Microcalorimetrv was used to analyze the thermogenic curves and parameters of Escherichia coli growth affected by zuofinwan and fanzuojin solution. In accordance with thermokinetic model, the relationships between k - c and I% - c of the drugs were fitted and IC50 were quantitated. The influence of the sequence of ＂ compatibility-decoction＂ on medical property of zuojinwan and fanzuojin was evaluated based on the literatures of ancient herbal. Results The growth of Escherichia coli was improved by decoctions of fanzuojin, however inhibited by zuojinwan. IC50 of zuojinwan in ＂ separated decoction-compatibility＂ group （5.63 mg · mL^-1 ） was lower than that of ＂ compatibility-combined decoction＂ group （7.40 mg· mL^-1 ）, which showed that the decoction of zuojinman in ＂ separated decoction-compatibility＂ group was more effective. Conclusion Microealorimetry can be used to evaluate the influence of the sequence of ＂compatibility-decoction＂ for rhizoma coptidis and fructus evodiae on the medical properly of zuojinu an and fanzuojin.