中文摘要：

目的 探讨肺腺癌细胞缺氧后胞红蛋白表达变化的特点与规律。方法 将人肺腺癌细胞株A549扩增后，分为4组：常氧组（对照组），缺氧4、12、24h组。对照组置常规培养箱（5％CO2、95％空气，37℃）中培养，各缺氧组置缺氧培养箱中培养（2％O2，5％CO2，93％N2，37℃）不同时间（4、12、24h）后，取肺腺癌细胞进行免疫组化染色和免疫印迹分析蛋白水平的分布与表达的变化，用逆转录PCR法分析胞红蛋白mRNA水平的变化。结果 免疫组织化学染色显示胞红蛋白主要分布于肺腺癌细胞的细胞浆，缺氧各组胞红蛋白染色强度均高于常氧组。免疫印迹灰度分析结果显示缺氧各组胞红蛋白表达量高于常氧组（153．4±7．1、233．3±26．8、276．2±41．4VS95．7±13．1．P〈0．05）。而缺氧12、24h组胞红蛋白表达高于缺氧4h组，差异有统计学意义（P〈0．05）。缺氧4、12、24h组mRNA水平均显著高于常氧组（85．6±15．4、136．2±27．4、148．8±20．1VS56．8±18．5，P〈0．05），缺氧12、24h组较缺氧4h时组更进一步增高（P〈0．05）。结论 胞红蛋白是分布于肺腺癌细胞的细胞浆中的一种珠蛋白。肺腺癌细胞缺氧4h后胞红蛋白表达上调，胞红蛋白表达增加可能是细胞对缺氧肿瘤的一种代偿反应。

英文摘要：

Objective To explore the characters of expression of cytolgobin in tumor cells after hypoxia. Methods Human pulmonary tumor cells of the line A549 were cultured and divided into 4 groups to be cultured under 5% CO2 and 95% air and exposed to 2% O2 ,5% CO2 ,and 93% N2 for 4, 12, or 24 hours respectively. The distribution of cytoglobin was examined by immunohistochemistry and the expression of cytoglobin was detected by Western blotting. The mRNA level of cytoglobin in the A549 cells was assayed by reverse-transcription PCR. Results Immunohistochemistry showed that cytoglobin was located in the plasma of the A549 cells, and the staining strength of cytoglobin was enhanced in the hypoxic groups in comparison with the normoxic group. Western blotting showed significantly stronger expression of protein of cytoblobin in the 3 hypoxic groups than in the normoxic group （ all P 〈 0.05 ）. The expression of cytoglobin was upregnlated significantly in the hypoxic 12- and 24-hour groups than in the hypoxic 4-hour group （ both P 〈 0.05 ）. The cytoglobin mRNA levels of the 3 hypoxic groups were all significantly higher than that of the normoxic group （ all P 〈 0.05 ）. The cytoglobin mRNA levels of the hypoxic 12- and 24-hour groups were significantly higher than that of the hypoxic 4-hour group （ both P 〈 0.05 ） , however, without a significant difference between the hypoxic 12- and 24-hour groups （P 〉 0.05 ）. Conclusion Hypoxia upregnlates the expression of cytoglobin in tumor cells.