中文摘要：

目的：探讨槐定碱在玻碳电极上的电化学反应机制及分析方法。方法：在硼酸-氯化钾-碳酸钠（A-P）缓冲溶液（pH7.4）中,采用循环伏安、计时电量、恒电位电解并结合紫外光谱法研究槐定碱在玻碳电极上的电化学反应机制,利用差示脉冲伏安法建立槐定碱的电化学分析方法。结果：槐定碱在玻碳电极上产生一不可逆的氧化峰,峰电位为0.875V,是两电子两质子的不可逆扩散氧化波,电荷转移系数为0.44,电极表观电子传递速率常数为7.12×10-3s-1;扩散系数为2.16×10-5cm2/s;其峰电流与槐定碱浓度在8.0×10-6～3.0×10-4mol/L范围内呈线性关系,检出限为2.0×10-6mol/L。结论：推导出了槐定碱的电极反应机制,可用于模拟生物样品中槐定碱的含量测定,结果满意。

英文摘要：

Aim：To research the electrochemical reaction mechanism of sophoridine（SR） and its electroanalytical application on the glassy carbon electrods.Methods：The electrochemical behavior of SR was studied on the glassy carbon electrods in A-P buffer solution （pH 7.4） by means of cyclicvoltammetry,chronocoulometry,bulk electrolysis with coulometry and UV spectrophotometry.Results：An oxidative peak of SR was achieved at 0.875 V. The results exhibited that the electrode reaction of SR was a diffusion-driven irreversible oxidative process involving two electrons and two protons. The charge transfer coefficient,appearance heterogeneous reaction rate constant,and diffusion coefficient was 0.44,7.12×10-3 s-1,and 2.16×10-5 cm2/s,respectively. The anodic peak current obtained by differential pulse voltammetry was proportional to the concentration of SR in the range of 8.0×10-6~3.0×10-4 mol/L. The detection limit was 2.0×10-6 mol/L.Conclusion：The electrochemical reaction mechanism of SR has been construed and the proposed method has been applied to the determination of SR in human sample with satisfactory result.