中文摘要：

目的研究授精前剥除部分卵丘细胞的卵母细胞的受精、胚胎发育和临床结局。方法授精前用机械法剥除卵母细胞外包裹的部分卵丘细胞，每个卵母细胞采用微滴法单独受精（A组）。对照组（B组）采用常规体外受精-胚胎移植（IVF-ET）受精方式进行。比较两组受精率（2PN、1PN和3PN）、卵裂率（2PN、1PN和3PN）、优胚率、临床妊娠率、种植率和流产率。结果A组和B组的受精率分别是76．51％和73．74％（2PN受精率分别是60．64％，61．62％；1PN受精率分别是1．61％，0．86％；3PN受精率分别是10．44％，9．69％）、卵裂率分别是98．95％和99．17％（2PN卵裂率分别是97．02，98．01％；1PN卵裂率分别是100％，85．71％；3PN卵裂率分别是100％，100％）、优质胚胎率分别是60．75％，62．68％、临床妊娠率分别是63．89％，61.76％、种植率分别是48．00％，38．78％、流产率分别是9．09％，11．90％。所有指标均无统计学意义。结论卵母细胞授精前剥除部分卵丘细胞不会影响IVF—ET的临床结局，为利用卵丘细胞进行非侵入性种植前遗传学诊断提供临床依据。

英文摘要：

Objective： To identify the effect of removal of cumulus cells prior to sperm-oocyte co-incubation on fertilization,cleavage and clinical outcome of in vitro fertilization（IVF）. Methods： The oocytes denuded by mechanical method were inseminated with 20,000 sperms per oocyte in 20 μ1 drops of IVF-30 covered by oil in group A. Two to five oocytes were fertilized with 100,000 sperms in 100 μ1 drops of IVF-30 covered by oil in group B. The fertilization rate including 2PN,1PN and 3PN, embryo cleavage rate including 2PN, 1PN and 3PN, high-quality embryo rate, clinical pregnancy rate, embryo implantation rate and abortion rate were compared between the two groups. Results： The fertilization rate was 76.51% vs. 73.74%（2PN fertilization rate is 60.64% vs. 61.62%, 1PN fertilization rate 1.61% vs. 0.86% ,3PN fertilization rate 10.44% vs. 9.69%） ,embryo cleavage rate 98.95% vs. 99.17% （2PN embryo cleavage rate 97.02% vs. 98.01% ,1PN embryo cleavage rate 100% vs. 85.71%,3PN embryo cleavage rate 100% vs. 100%）, high-quality embryo rate 60.75% vs. 62.68%,clinical pregnancy rate 63.89 % vs. 61.76 %, embryo implantation rate 48.00 % vs. 38.78 %, and abortion rate 9.09% vs. 11.90% in group A and group B respectively. The parameters were no statistically differences between the two groups. Conclusions. Removal of cumulus cellsprior to IVF has no adversely effect on the IVF-ET clinical outcome. The results provide the clinical basis for using cumulus cells to do non-invasive preimplantation genetic diagnosis.