中文摘要：

基于纳米生物共轭的酶活探针由于纳米材料的独特光电及电化学性质，多年来已经进行了广泛的研究。本综述总结了纳米金生物共轭探针在检测酶活性方面的研究成果，依次讨论了比色法、荧光法以及其他的方法，对其独特的设计进行了解释并讨论了各方法的优缺点。最后，简要指出了其发展的方向以及成功应用于实际应用时所存在的障碍。

英文摘要：

The enzymatic probe based on nano-bioconjugate have been studied extensively for many years for their novel electronic, photonic and electrochemical properties. This review summarizes major advances in the gold nanoparticles（GNPs） biocongjugate probes to detect enzyme activity. This is followed by a discussion of the assays in colorimetric, fluorescence and other assays. Throughout the review, a detailed explanation of the unique designs will be presented, and the benefits and shortcomings of these approaches will be highlighted. The review concludes with a brief perspective on future research directions, and remaining barriers that must be overcome for the successful application of these technologies.