中文摘要：

流上皮的功能的动态平衡的上皮的房间和损失是的航线气喘的主要病理学的特征；然而，位于这些病理下面的机制仍然保持阴暗。我们的以前的工作证明在 integrin 的 5 flanking 区域有三个变化地点 4 在气喘病人，它与 integrin 的减少的表示被相关 4 在外部白血球。Integrin 4 是航线 epithelia 上的一个重要结构的粘附分子保留上皮的房间的结构的粘附。在这个工作，我们进一步表明了 4 表示是的那 integrin 在气喘病人的航线 epithelia 的 downregulated。探查在 integrin 的 imbalanced 表示之间的关系 4 并且航线的机能障碍在气喘的上皮的房间， integrin 4 由 integrin 是在人的支气管的上皮房间(16HBE14O ) 的 silenced 4 小介入的 RNA lentivirus 向量。在 integrin 的 silencing 之上 4， 16HBE14O 房间显示出减少的增长和创伤修理。大多数房间被显示在 integrin 以后在 G1 阶段被逮捕 4 silencing，和增加的 apoptosis 在 integrin 4 沉默房间被导致。在摘要，我们的结果提供了引人注目的证据那 integrin 4 涉及结构的正直和航线的功能的动态平衡上皮的房间。它是可能的 4 在气喘航线 epithelia 上贡献的 integrin 的那 downregulation 航线的结构的混乱和机能障碍上皮的房间。

英文摘要：

The shedding of airway epithelial cells and loss of epithelial functional homeostasis are major pathological characteristics of asthma; however, the mechanism underlying these pathologies remains obscure. Our previous work showed that there were three variation sites in 5＇ flanking region of integrin β4 in asthma patients, which was correlated with decreased expression of integrin β4 in peripheral leukoeytes. Integrin β4 is an important structural adhesion molecule on airway epithelia to keep the structural adhesion of epithelial cells. In this work, we further demonstrated that integrin β4 expression was downregulated in airway epithelia of asthma patients. To probe the relationship between imbalanced expression of integrin β4 and dysfunction of the airway epithelial cells in asthma, integrin β4 was silenced in human bronchial epithelium cells （16HBE140） by integrin β4 small-interfering RNA lentivirus vector. Upon silencing of integrin β4, 16HBE140 cells showed reduced proliferation and wound repair. Most cells were shown to be arrested in G1 phase after integrin β4 silencing, and increased apoptosis was induced in the integrin β4-silenced cells. In summary, our results provided compelling evidence that integrin β4 was involved in the structural integrity and functional homeostasis of airway epithelial ceils. It is likely that downregulafion of integrin β4 on asthma airway epithelia contributes to the structural disruption and dysfunction of airway epithelial cells.