中文摘要：

目的：采用小鼠成肌细胞C2C12作为模型，观察光生物调节作用对他汀类药物引起的肌病的作用。 方法：实验于2004-09／2005-01在华南师范大学激光运动医学实验室完成。C2C12细胞用浓度分别为2.0×10^-5, 2.0×10^-6, 2.0×10^-7 ，2．0×10^-8 mol／L的辛伐他汀培养，然后用强度分别为0，0．229，0．506，0．848，1．401，1．670mW／cm^2的红色发光二极管[波长（640±15）nm]照射2d，15min／d。用甲基噻唑基四唑比色法评价细胞增殖。 结果：浓度为2.0×10^-6, 2.0×10^-7 and 2.0×10^-8的辛伐他汀对C2C12的增殖没有影响，无光生物调节作用；浓度为2．0×10^-5 mol／L的辛伐他汀抑制C2C12的增殖，发光二极管强度为0，0．229，0．506，0．848，1．401，1．670mW／cm^2时C2C12细胞增殖吸光度百分率分别降为（37．2±84）％，（58．4±94．9）％，（37．0±8．6）％，（63．0±8．8）％，（59．2±12．6）％，（28．9±90．3）％。强度为0．848mW／cm^2的红色发光二极管照射2d，15min／d可促进被抑制的C2C12增殖效应。 结论：红色发光二极管可以促进被辛伐他汀抑制的C2C12细胞的增殖作用，对服用他汀类药物引起的肌病可能有光生物调节作用。

英文摘要：

AIM： To use the mouse myoblast line, C2C12, as an in vitro model to observe the effect of photobiomodulation on statin induced myopathy. METHODS： The experiment was carried out at the Laboratory of Laser Sports Medicine, South China Normal University, between September 2004 and January 2005. C2C12 cells were cultured with simvastatin at 2.0×10^-5, 2.0×10^-6, 2.0×10^-7 and 2.0×10^-8 mol/L and irradiated by red light emitting diode （RLED） [640±15 nm in wave length] at 0, 0.229, 0.506, 0.848, 1.401 and 1.670 mW/cm^2 separately for 15 minutes per day, 2 days in total. The cellular proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyl tetrazolium bromide （MTT）. RESULTS： Simvastatin at 2.0×10^-6, 2.0×10^-7 and 2.0×10^-8 mol/L did not inhibit C2C12 cellular proliferation, and no photobiomodulation was found. However, simvastatin at 2 ×10^ -5 mol/L inhibited C2C12 cellular proliferation . When the intensity of RLED was 0,0.229,0.506,0.848, 1.401,1.670 mW/cm^2, the absorbance of C2C12 cellular proliferation was （37.2±8.4）%, （58.4±24.9）%, （37.0±8.6）%, （63.0±8.8）%, （59.2±12.6）%, （28.9±20.3）%, respectively . The inhibited C2C12 proliferation was promoted with RLED irradiation at 0.848 mW/cm^2 for 15 minutes per day, 2 days in total. CONCLUSION： RLED can promote the proliferation of C2C12 inhibited by statin, which indicates that RLED might .has photobiomodilative effect on statin induced myopathy.