中文摘要：

目的：研究hsa-miR-21-3p对人食管癌细胞Eca9706侵袭和迁移能力的影响。方法：采用终浓度为30 nmol/L的寡核苷酸hsa-miR-21-3p mimics和阴性对照转染Eca9706细胞48 h后，用实时荧光定量PCR检测转染后hsa-miR-21-3p的表达水平，通过Transwell体外侵袭试验和划痕愈合试验分别观察hsa-miR-21-3p对Eca9706细胞侵袭和迁移能力的影响。结果：与阴性对照组相比4较，hsa-miR-21-3p mimics转染组细胞中hsa-miR-21-3p表达明显升高（P＜0.01），是阴性对照的1.1×10^4倍；Transwell体外侵袭试验结果显示镜下每个视野穿膜细胞数试验组（76.67±6.11）较阴性对照组（42.33±2.52）增加了81.1%（P＜0.01），表明hsa-miR-21-3p的过表达显著增强食管癌细胞的侵袭能力；划痕愈合试验结果显示，hsa-miR-21-3p试验组的划痕宽度较对照组窄，在相同的时间点，试验组的划痕愈合能力均大于对照组（P＜0.01），说明转染了hsa-miR-21-3p mimics的试验组细胞的迁移能力大于对照组。结论：Hsa-miR-21-3p的高表达可以使人食管癌细胞Eca9706的侵袭和迁移能力增强，提示其可能参与了食管癌进程中的浸润和转移。

英文摘要：

OBJECTIVE：To explore the potential role of hsa-miR-21-3p in invasion and migration ability of esophageal carcinoma cell line Eca9706. METHODS：Quantitative real-time PCR was used to evaluate the expression level of miR-21-3p in Eca9706 cells and control transfected with 30 nmol/L of hsa-miR-21-3p mimics after 48 h. Transwell and wound healing experiment were carried out to determine cell invasion and migration ability of Eca9706 cells transfected with hsa-miR-21-3p mimics. RESULTS：Hsa-miR-21-3p expression level in hsa-miR-21-3p mimics-4transfected Eca9706 was increased 1.1×10^4 times compared with negative control. Transwell assay showed the number of transmembrane cells in experimental group was increased 81.1% compared with negative control,suggested that over-expression of hsa-miR-21-3p enhanced the invasion ability of Eca9706 cells. Wound healing experiment demonstrated the capacity of Eca9706 cells migration was strengthened after transfection with hsa-miR-21-3p mimics. CONCLUSION：Up-regulation of hsa-miR-21-3p might serve as an oncogene and participate in the invasion and metastasis of esophageal cancer.