中文摘要：

目的 研究孕期炎症刺激剂脂多糖（lipopolysacchar-ides,LPS）刺激对子代大鼠6周龄时血管结构的影响。方法12只Sprague dawley（SD）孕鼠随机分为3组：对照组（control,Con）、LPS刺激组、LPS刺激加二硫代氨基四氢呋喃（pyrrolidinedithiocarbamate,PDTC）干预组（L＋P）,分别在孕8-14d腹腔注射生理盐水、LPS（0.79mg·kg^-1）或LPS加PDTC（100mg·kg^-1）,LPS于孕d8、10、12给药;生理盐水及PDTC于d8-14每天给药。子代出生6周测体质量;透射电镜观察血管超微结构;实时荧光定量PCR检测仔鼠胸主动脉连接蛋白（connexin,Cx）Cx37、Cx40、Cx43、Cx45的mRNA表达水平;Western blot和免疫荧光检测仔鼠胸主动脉Cx37、Cx40、Cx43、Cx45的蛋白表达水平。结果 与Con组相比,LPS组子代大鼠在6周时♂♀体质量均明显增加（P〈0.01）;L＋P组♂鼠明显低于LPS组（P〈0.05）,而♀鼠差异无显著性。LPS组内皮受损,内皮细胞间可见明显缝隙连接（gapjunction,GJ）,相对于Con组较长且多见;L＋P组内皮损伤程度较LPS组稍有改善。LPS组Cx43 mRNA及蛋白表达均明显低于Con组（P〈0.05）,L＋P组则明显高于LPS组（P〈0.05）;Cx37、Cx40和Cx45各组间mRNA及蛋白表达差异无统计学意义。LPS组Cx43荧光强度及荧光点数明显比Con组降低,而L＋P组明显比LPS组高,各组间Cx37、Cx40和Cx45荧光强度和荧光点数并无明显差异。结论孕期LPS刺激可致子代大鼠在6周龄时出现血管结构损伤,这种损伤可能持续于新生儿期,并与其成年发生高血压密切相关。

英文摘要：

Aim To explore the role of prenatal exposure to lipopolysaccharides（LPS）on aortic morphology in the 6-week offspring rats.Methods Twelve pregnant rats were randomly divided into three groups： control group, LPS group, L＋P（pyrrolidinedithiocarbamate,PDTC）group.The rats were intraperitoneally administered vehicle, LPS（0.79 mg·kg^-1）,or LPS plus PDTC（100 mg·kg^-1）.LPS was givenon the 8th,10th and 12th day, where as vehicle and PDTC were given daily from the 8th to the 14th day during gestation.Offspring body weight was measured at 6-week, and histopathological alteration of the thoracic aorta was observed by transmission electron microscopy, the thoracic aorta mRNA and protein expression of connexin（Cx）molecules including Cx37, Cx40,Cx43 and Cx45 in offspring rats were detected by real time PCR,Western blot and confocal laser-scanning microscope.Results Body weight at 6-week offspring rats was significantly higher in LPS group than in control group whatever male or female（P〈0.01）; and that in L＋P group was significantly lower than in LPS group in male rats（P〈0.05）,but not in female rats.The LPS group thoracic aortas exhibited lesions, including impaired endothelial cells, and the vascular smooth muscle cells were arranged disorder and migration and proliferation; the number of gap junction was increased and longer than control group; the pathological changes were improved to some extent in L＋P group.Cx43 mRNA and protein expression in LPS group was obviously lower than in control group（P〈0.05）, which could be partly reversed in L＋P group（P〈0.05）.There was no significant difference in Cx37,Cx40 or Cx45 mRNA or protein expression among each group.The results of protein expression with confocal laser-scanning microscope was the same as those of Western blot.Conclusion Maternal LPS exposure during pregnancy leads to vascular changes in 6-week offspring rats, which might last until neonatal stage, and is closely correlated to hypertension in adult offsprings.