中文摘要：

目的 研究孕期炎症刺激对子代大鼠新生儿期血管结构的影响.方法 12只Sprague Dawley孕鼠采用随机数表法分为3组,即对照组、脂多糖（lipopolysaccharides,LPS）刺激组（LPS组）和LPS刺激＋二硫代氨基四氢呋喃（pyrrolidinedithiocarbamate,PDTC）干预组（PDTC组）,分别在孕8 ～14 d腹腔注射生理盐水、LPS（0.79 mg/kg）或LPS＋ PDTC（100 mg/kg）.分别于子代大鼠出生后1d和1周测体质量,组织切片观察子代大鼠胸主动脉病理形态变化,透射电镜观察血管超微结构,实时荧光定量PCR检测子代大鼠胸主动脉连接蛋白（connexin,Cx）37、Cx40、Cx43、Cx45的mRNA表达水平,免疫荧光检测子代大鼠胸主动脉Cx37、Cx40、Cx43、Cx45的蛋白表达水平.结果 LPS组子代大鼠1天龄及1周龄时体质量均明显低于对照组[分别为（6.742±0.128）g 比（7.425 ± 0.146）g,和（13.264±2.581）g 比（20.173 ±3.982）g,P均＜0.01],PDTC组[分别为（7.137 ±0.141）g和（17.863 ±3.412）g]则均明显高于LPS组（P均＜0.01）.LPS组子代大鼠1周龄时内皮层受损、平滑肌细胞增殖迁移,中膜较对照组厚,缝隙连接（gap junction,GJ）较对照组少,PDTC组与LPS组比较无明显差别.LPS组子代大鼠1天龄和1周龄时胸主动脉Cx43的免疫荧光表达明显低于对照组,PDTC组则明显高于LPS组;各组间Cx37、Cx40和Cx45的免疫荧光表达无明显差异.LPS组子代大鼠1天龄及1周龄时胸主动脉Cx43的mRNA表达水平均明显低于对照组（分别为1.226 ± 0.209比1.530 ±0.296和7.204± 1.165比9.357 ± 1.917,P均＜0.05）,PDTC组（分别为1.619 ±0.324和9.271 ± 1.514）则均明显高于LPS组（P均＜0.05）;Cx37、40、45的mRNA表达水平各组间差异则无统计学意义.结论 孕期炎症刺激可导致子代大鼠新生儿期血管结构损伤,这可能与其成年发生高血压相关.

英文摘要：

Objective To explore the role of prenatal exposure to lipopolysaccharides LPS） on aortic morphology in the neonatal offspring rats.Methods Twelve pregnant rats were randomly divided into three groups：control group,LPS group,and PDTC （pyrrolidinedithiocarbamate,LPS ＋ PDTC） group.The rats were intraperitoneally administered vehicle,LPS （0.79 mg/kg）,or LPS plus PDTC （100 mg/kg）,respectively.LPS was given on the 8th,10th and 12th days,whereas vehicle and PDTC were given daily from the 8th to the 14th day during gestation.Histopathological alteration of the thoracic aorta was observed by hematoxylin-eosin staining and transmission electron microscopy,thoracic aortic mRNA and protein expression of connexin （Cx） molecules including Cx37,Cx40,Cx43 and Cx45 in offspring was detected by Real Time PCR and confocal laser-scanning microscope,respectively,offspring body weight was measure at day 1 and week 1.Results Body weight at 1 day and 1 week-old offspring was significantly lower in LPS group than in control group （P 〈 0.01）,which were significantly higher in PDTC group compared to LPS group （P〈0.01）：[1 day：control group （7.425 ±0.146） g,LPS group （6.742 ±0.128） g,PDTC group （7.137 ±0.141） g; 1 week：control group （20.173 ±3.982） g,LPS group （13.264 ±2.581） g,PDTC group （17.863 ± 3.412） g].In 1 week-old offspring of LPS group,the thoracic aortas exhibited lesions,including impaired endothelial cells,thickening and fibrous changes of intimae,and migration and proliferation of vascular smooth muscle cells; the number of gap junction was decreased versus control group and pathological changes were similar between PDTC group and LPS group.Cx43 protein expression in LPS group was obviously lower than in control group and which could be partly reversed in PDTC group.Expression of Cx43 mRNA was significantly lower in 1 day and 1 week offspring of LPS group compared to control group （P 〈 0.05）,which could be reversed in PDTC group （P 〈 0.05） （1 day