采用固相微萃取(SPME)和GC-MS分析研究木枣和酸枣挥发物成分,并测试枣镰翅小卷蛾触角电位(EAG)反应、寄主趋向和产卵选择,以探明枣镰翅小卷蛾的寄主选择机制。结果显示,在萌芽期,木枣和酸枣嫩叶的挥发成分均为罗勒烯、2-甲基-2-菠烯、α-法呢烯和邻苯二甲酸二丁酯4种,但相对含量稍有不同。枣镰翅小卷蛾成虫对木枣和酸枣2种寄主都有强烈的EAG反应,且同一寄主上雌蛾的EAG反应值极显著地高于雄蛾,其EAG值是雄蛾的3.3倍。枣镰翅小卷蛾对木枣表现强烈的趋向反应,而对酸枣的趋向不明显,且雌虫的趋向反应显著高于雄虫。木枣上的产卵量显著高于酸枣,且木枣枣吊上的单雌产卵量为307.9粒,极显著地高于酸枣枣吊上的产卵量(182.9粒)。研究表明,枣镰翅小卷蛾雌蛾在寄主选择中起主导作用,木枣是其嗜好寄主。
In order to define the mechanism of host selection of jujube armyworm adult,Ancylis sativa Liu,the volatile chemical components from Muzao,Zizyphus jujuba and sour jujube,Z.spinosa,were analyzed using solid phase microextraction(SPME) and GC-MS.The EAG response,host and oviposition selection of adult A.sativa were tested with EAG and Y-olfactormeter.The results indicated that four components,ocimene,2-Methyl-2-bornene,α-Farnesene and dibutyl phthalate,were identified in the volatile from Muzao and sour jujube during the sprouting period,but the relative contents of four components were different in two hosts.EAG response(millivolts) of adult A.sativa to the sprout of Muzao and sour jujube showed strongly high.In same host,the EAG response of female adult antenna was 3.3 times of male(P0.01).The adults were intensely attracted by Muzao,but they were not obviously attracted by sour jujube.Meanwhile,the female adults were attracted more strongly than the male.A test of oviposition selection of A.sativa showed that eggs were obtained on Muzao more than sour jujube under controlled condition.The mean number of eggs per female laid on Muzao leaves was 307.9,which is significantly higher than those on sour jujube(182.9).The female played an important role in host-plant selection of A.sativa,and Muzao was preferred host of the armyworm compared to sour jujube.