在未经灭菌的Zarrouk培养基中分别添加不同浓度的谷氨酸钠和葡萄糖,考察其对螺旋藻生长的影响。结果表明:葡萄糖的最佳添加浓度为3g/L,螺旋藻中的叶绿素含量和藻胆蛋白含量分别是对照的1.9倍和1.13倍;在3g/L葡萄糖浓度下,考察不同浓度的谷氨酸钠对螺旋藻混合营养培养的影响,以干重、叶绿素及藻胆蛋白含量、藻液的污染程度为主要指标,得出1.2g/L为最佳添加量。
The effects of sodium glutamine with different concentrations on mixotrophic culture of S. platensis were studied. The results showed that when 3 g/L glucose is appended, the content of chlorophyll is 1.9 times of that in control, but the content of phycobiliprotein is only 1.13 times. At the fixed concentration of 3 g/L glucose, among the sodium glutamine concentrations 0, 0.2, 0.6, 0.9, 1.2, 1.5 and 2 g/L, 1.2 g/L is the best, according to the standards of the cell dry weight, the contents of chlorophyll and phycobiliprotein, pollution level of S. platensis.