中文摘要：

本实验利用自制的光生物反应器进行螺旋藻混合营养培养，分别采用单因子试验和因子分析设计对添加的混合营养成分进行筛选。结果表明，影响螺旋藻生长的主要因子是葡萄糖、硝酸铵和VB1。利用响应面法（RSM）对上述三个显著因子的最佳水甲范围进行研究，并建立以藻体干重（DW）为响应值，以葡萄糖、硝酸铵和VBl为自变量的二次多项式数学模型。最后对光照强度和营养成分的不同补加周期进行优化研究，最终藻体干重为1．822g／L。

英文摘要：

In this study, a self-made bioreactor was employed for mixotrophic culture of Spirulina. The important factors influencing growth of Spirulina were screened out by single-factor test and factional factorial design, which are added concentrations of glucose, NH4NO3 and VB1. The response surface methodology was used to optimize the above three factors. Based on the results of optimization test, a quadratic regression equation was built up with dry weight as the response value and with added concentrations of glucose, NH4NO3 and VB1 as independent variables. Finally, the light intensity and the added cycles of glucose, NH4NO3 and VB1 were optimized. Under the optimized conditions, the dry weight of Spirulina is up to 1.822 g/L.