本研究以4~8周龄的辽宁绒山羊母羔为研究对象,研究不同激素处理对羔羊卵泡发育数量和发育质量的影响,同时还比较了不同配方的培养液对羔羊卵母细胞体外成熟率的影响。结果表明:最佳的母羔超数排卵方案是依次注射PMSG 400 IU 1次,FSH(加拿大)40 mg 3次,间隔时间12 h;在成熟培养液中添加15%的发情羊血清(ESS)卵母细胞的体外成熟率最高。
In this study, 4~8 week-old Liaoning cashmere goats female lamb were used for the research of different hormone treatment on ferent medium formulations for lamb oocyte follicular development quantity and quality. And Dif- in vitro maturation rate were discussed as well. The results showed that: the best female lambs PMSG 400 IU for 1 time, and FSH (Canada) 40 maturation experiment, add 15% (v/v) of the highest in vitro maturation rate. superovulation program is followed by injection of mg for 3 times with intervals of 12h each. In oocytes estrous goat serum (ESS) in maturation medium got the