离子液因其具有良好的生物兼容性和独特的理化性质,近年来在生物催化和生物大分子蛋白质与核酸的分离分析领域得到广泛应用。离子液与生物大分子相互作用的研究是离子液相关理论与应用研究的基础,有关离子液与蛋白质和核酸相互作用的机理研究受到关注。本文简要介绍了常用离子液的分类,离子液与蛋白质分子作用的机理,离子液与核酸分子作用的机理,以及离子液在酶催化反应、生物分子分离、生物分子电化学分析和毛细管电泳分析中的应用,并主要综述了近年的相关研究和应用进展。
Room-temperature ionic liquids(ILs)have aroused considerable interest recent years.They have been widely applied in biological catalysis and proteins and nucleic acids separation due to their good compatibility and unique properties.The interaction of ionic liquids and biological macromolecules is the foundation of their theory and application study.In this paper,the types of common used ILs,the principle of interaction between ionic liquids and protein,and ionic liquids and nucleic acid are introduced respectively.Furthermore,the recent application of ILs in biological catalysis,biological molecules separation electrochemical analysis as well as capillary electrophoresis analysis is reviewed.