中文摘要：

利用毛细管电泳激光诱导荧光（CE-LIF）表征了偶联剂N-羟基硫代琥珀酰亚胺（NHS）及NHS和1-（3-二甲氨基丙基）-3-乙基碳二亚胺盐酸盐（EDC）混合偶联剂对CdTe量子点活化效果的差异,优化了CdTe与转铁蛋白（Trf）的偶联条件,比较了CdTe-Trf,CdTe-BSA偶联物及CdTe量子点对Hela细胞的标记效果.结果表明：NHS活化后的量子点性能优于混合偶联剂EDC-NHS.31.2 μmol/LTrf与CdTe形成的偶联物CdTe-Trf对Hela细胞的标记效果最佳.CdTe-Trf在4℃孵育20 min可快速标记在Hela细胞的表面；在37℃孵育7h后,可进入Hela细胞内.说明所制得的CdTe-Trf偶联物具有Trf的生物功能,它可通过特异性识别并结合细胞膜表面的Trf受体而转入Hela细胞.而CdTe-BSA偶联物的标记不具特异性,CdTe则不能用于Hela细胞的标记.

英文摘要：

Capillary electrophoresis-laser inductive fluorescence （CE-LIF） was used to characterize the active effect difference of coupling agent N-hydroxysulfo-succinimide （NHS） and the mixture of NHS and 1-ethyl-3-（3-dimethylaminopropyl） carbodiimides hydrochloride （EDC） on quantum dot CdTe, and to opti- mize the coupling conditions of CdTe and transferrin （Trf）. The labeling effect of CdTe-Trf and CdTe-BSA complex as well as free CdTe for Hela cell were compared. Results demonstrated that the property of CdTe activated by NHS was better than the mixture of EDC-NHS. When 12.5, 31.2 and 40.6 p.mol/L Trf were used to couple CdTe respectively, the resultant of CdTe with 31.2 p~mol/L Trf optimized by CE-LIF showed the best labeling on Hela. The CdTe-Trf resultant labeled Hela rapidly on surface when they incubated at 4 ~C for 20 min, and transferred into Hela when they incubated at 37℃ for 7 h, which manifested the CdTe-Trf resultant had biological function. The CdTe-Trf can recognize and bind the Trf receptor on cell membrane specifically and enter Hela, while CdTe-BSA can not recognize Hela specifically and free CdTe can not label on cell.