中文摘要：

以改性壳聚糖和纳米金复合物为电极修饰材料,包埋固定黄曲霉毒素B_1抗体制备信号放大型免疫传感器。以K_3[Fe（CN）_6]为探针,利用循环伏安法和差分脉冲伏安法研究其免疫反应对传感器响应电流的影响,结果表明,免疫响应电流与溶液中AFB_1的浓度在0.1-1.1 ng/m L范围内成线性关系,最低检测限为0.05 ng/m L（S/N=3）;免疫传感器的特异性、稳定性和重现性较好。利用该法对花生、花生油等实际样品中的黄曲霉毒素B_1进行检测,回收率在87.8-98.2%,检测精确度优于ELISA试剂盒,可用于食品中黄曲霉毒素B_1的快速检测。

英文摘要：

A nano immunosensor for highly sensitive determination of aflatoxin B1 in food has been constructed by the modified composite （ chitosan and gold nanoparticles ） immobilization of aflatoxin B1 antibody on glass carbon electrode. The effects of immune response on the immunosensor response current were investigated by cyclic voltammetry and differential pulse voltammetry using K3[Fe （ CN ） 6] as the probe. The results showed that the immune response currents increased linearly with the concentration of aflatoxin Bt in the range of 0.1-1.1 ng/mL with detection limit 0.05 ng/mL （ S/N=3 ） ; Excellent specificity, stability and reproducibility of the prepared CS-GNPs/AFB1-Ab immunosensor was observed in the selected condition. The developed method was applied to the determination of aflatoxin B1 in samples such as peanut and peanut oil, and the results well agreed with those obtained by enzyme-linked immunosorbent assays method. Being more precise and accurate than ELISA method, it was practicable for rapid determination of aflatoxin B1 in vegetable oils with simple pretreatment.