中文摘要：

目的观察枸橼酸铁铵（FAC）对细胞外α-突触核蛋白（α-syn）作用的C6胶质瘤细胞释放炎性因子的影响。方法分别用α-syn、FAC、α-syn＋FAC处理C6细胞,以无血清培养液孵育C6细胞作为对照组,采用实时荧光定量PCR方法检测各组细胞白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）mRNA表达。结果α-syn组细胞IL-1β、TNF-α的表达较对照组显著增加（F=5.43～67.19,q=4.07～16.25,P〈0.05）,10μmol/L FAC组细胞二者的表达与对照组比较无明显变化（P〉0.05）,100μmol/L FAC组二者的表达较对照组显著增加（q=14.48、4.14,P〈0.05）,α-syn＋FAC组IL-1β、TNF-α的表达与α-syn组比较无明显变化（P〉0.05）。结论外源性α-syn可以促进C6细胞释放炎性因子,高铁对α-syn引起的炎性因子释放无明显影响。

英文摘要：

Objective To examine the effect of ferric ammonium citrate（FAC）on the release of pro-inflammatory cytokines by C6 glioma cells exposed to extracellularα-synuclein（α-syn）. Methods C6 cells were stimulated withα-syn,FAC,α-syn＋FAC,or serum-free medium（control）.The mRNA expression of intracellular interleukin-1β （IL-1β ）and tumor necrosis factor-α（TNF-α）was measured using real-time PCR. Results The IL-1β and TNF-αexpression in theα-syn group was significantly higher than that in the control group（F=5.43-67.19,q=4.07-16.25,P〈0.05）.On the other hand,while there were no significant differences in IL-1β and TNF-αexpression between the 10μmol/L FAC group and the control group（P〈0.05）,IL-1β and TNF-αexpression was significantly enhanced in the 100μmol/L FAC group compared with the control group（q=14.48-4.14,P〈0.05）.However,there were no significant differences in IL-1β and TNF-αexpression between theα-syn＋FAC group andα-syn group（P〈0.05）. Conclusion Extracellularα-syn can stimulate C6 cells to release pro-inflammatory cytokines,which is not affected by the increase in iron levels.