中文摘要：

目的观察弓形虫排泄分泌抗原（ESA）与可溶性速殖子抗原（STAg）联合或单独滴鼻免疫小鼠的免疫应答效果，筛选弓形虫黏膜疫苗候选抗原。方法将40只BALB／c小鼠随机分为4组，每组10只。实验组分别用20μg STAg、30腭ESA及二抗原联合（15μgESA与10μgSTAg）滴鼻免疫2次，间隔14d，对照组用PBS以20μg，只滴鼻。于末次免疫后第14天，取血清，收集小肠冲洗液，检测特异性抗体，分离肠上皮内淋巴细胞（iIEL）和脾淋巴细胞并计数。结果整个实验期间，各组小鼠健康状况良好，体重逐渐增加。STAg组、ESA组及联合抗原组小鼠黏膜、血清中特异性抗体水平依次升高，脾淋巴细胞和iIEL增殖反应逐渐增强。联合抗原组和ESA组血清I赆抗体和小肠冲洗液sIgA升高尤为显著。同时，联合抗原组、ESA组脾淋巴细胞和iIEL增生显著高于对照组。结论ESA单独或联合STAg滴鼻免疫小鼠均能激发弓形虫特异性黏膜及系统免疫应答。两种抗原联合免疫的研究，将有助于制定更加完善的弓形虫疫苗研究策略。

英文摘要：

Objective To observe the immune responses induced by intranasal immunization with either of excreted/secreted antigens （ESA） and soluble tachyzoite antigen（STAg）as well as combined immunization with the two antigens and screen the candidate antigen for preparation of mucosal vaccine agmnst Toxoplasma gondii .Methods Divide 40 BALB/c mice into 4 groups, 10 for each. The mice in test groups 1, 2 and 3 were immunized twice with 20 μg STAg,30 μg of ESA and 15 μg of ESA pins 10 μg of STAg by intranasal drip respectively,at interval of 14 d, and those in control group with 20 μl of PBS. Kill the mice 14 d after the 2rid immunization and collect sera and intestial washes for determination of specific antibody, and isolate intestinal intraepithelial lymphocytes（iIEL） and spleen lymphocytes for counting. Results During the whole study, the mice in each group were healthy, and their bodyweights increased gradually. In order of test groups 1,2 and 3, the specific antibody levels in sera and intestinal washes increased, and iIEL and spleen lymphncytes increased. The IgG levels in sera and sIgA levels in intestinal washes of test groups 2 and 3 increased remarkably, and their iIEL and spleen lymphocyte counts were significantly higher than those in control group. Conelttsion Either immunization with ESA or combined immunization with ESA and STAg by intranasal drip induced mucosal and systemic immune response against Toxoplasma gondii. The study on combined immunization with ESA and STAg was helpful to development of new strategy of Toxoplasma gondii vaccination.