中文摘要：

[目的]对从自然界得到的1株乳白耙齿菌菌株CB1（简称Il-CB1）进行系统发育分析与鉴定,并研究其对刚果红染料的脱色条件,以期为乳白耙齿菌在染料废水脱色中的应用提供依据。[方法]在形态学鉴定的基础上,克隆菌株Il-CB1的ITS序列并进行系统发育分析。采用单因素方法检测p H值、刚果红浓度、染料加入时间对Il-CB1脱色率的影响;基于正交试验检测碳源浓度、氮源浓度、Cu^2＋浓度、Mn^2＋浓度、接种量5个因素对Il-CB1脱色率的影响。[结果]菌株I1-CB1的ITS序列与其他不同来源的24个乳白耙齿菌的ITS序列归为一类,从而在分子水平上鉴定其为乳白耙齿菌。p H值对Il-CB1脱色刚果红的影响：脱色率随p H值增加先升高后降低,p H=3和8时脱色率均较低,其脱色刚果红的最适p H=6.0。刚果红浓度对Il-CB1脱色的影响：在液体条件下的脱色率随刚果红浓度的升高呈先升高后降低的趋势,对100 mg·L^-1的刚果红脱色率最高,当继续升高染料浓度为250 mg·L^-1时,脱色率明显下降,表明较低浓度的刚果红促进Il-CB1对其脱色,而高浓度的刚果红对Il-CB1脱色产生抑制。正交试验表明,Il-CB1对刚果红脱色的最优因素组合为：葡萄糖10 g·L^-1、蛋白胨3 g·L^-1、Cu^2＋和Mn^2＋的添加浓度均为0.2 mmol·L^-1、在250 m L三角瓶中装液量为100 m L的条件下接种量为7 m L菌丝母液,在以上条件下第5天时染料脱色率高达99.60%。在上述所有培养条件优化的基础上,染料加入时间对Il-CB1脱色刚果红的影响：与0 h加入刚果红染料相比,在菌株培养3天后加入刚果红染料,其脱色时间明显缩短,初始脱色率就处于较高水平,在菌株培养3天时加入刚果红染料处理5天后的脱色率高达99.99%。[结论]菌株Il-CB1为乳白耙齿菌,其可有效降解刚果红染料,在染料废水降解中具有重要的应用潜能。

英文摘要：

[Objective]Strain Irpex lacteus CB1（Il-CB1） was identified firstly and its decoloring conditions to congo red was systematically studied so as to prove its application potentials in dye decolorization.[Method ]On the basis of morphological identification,the ITS sequence of Il-CB1 was cloned and its phylogenetic analysis was made.The effects of p H value,congo red concentration,and adding time of dye on decoloring congo red by Il-CB1 were tested through single factor experiments,and the effects of other five factors including carbon concentration,nitrogen concentration,Cu^2＋and Mn^2＋concentration and inoculum volume on decolorization of Il-CB1 were studied through orthogonal experiment.[Result]The ITS sequence of Il-CB1 and other 24 ITS sequences from different I.lacteus strains clustered together,indicating that Il-CB1 was I.lacteus.The effect of p H value on congo red decolorization showed that decolorization percent increased first and then decreased with the increase of p H value.The decolorization percent both was very low under p H 3and 8,the optimum p H value was 6.0.Decolorization percents under different congo red concentrations increased first and then decreased with the increase of congo red concentration.Decolorization percent under 100 mg·L^-1congo red was the highest,and it decreased significantly under 250 mg·L^-1congo red,indicating that lower congo red concentrations promoted decolorization,while higher congo red concentration inhibited decolorization.Orthogonal experiment showed that the most optimal culture parameters to decolorization were：glucose 10 g·L^-1,peptone 3 g·L^-1,Cu^2＋and Mn^2＋both 0.2mmol·L^-1,7 m L inoculum volume of mycelium liquor in 100 m L culture solution bottled in 250 m L flask.Decolorizationpercent was 99.60% on 5 d under above-mentioned conditions.Decolorization at different adding time of congo red was tested based on all above optimal culture conditions.The initial decolorization percent remained at a high level and it was up to 99.99% on 5 d when con