中文摘要：

地黄连作障碍严重制约了地黄的产量和品质,化感自毒作用是植物连作障碍形成的重要原因之一,研究地黄化感物质的来源（根、叶、根系分泌物）显得尤为重要.通过在土壤中添加地黄化感物质的不同载体（根、叶、根系分泌物）,对一段时期内土壤微生物功能多样性发生的变化进行分析,基于BIOLOG平板法分析不同时期各个处理以酚酸类物质为碳源及以其他碳源为底物的微生物生长情况,研究化感物质的主要来源.结果表明：处理1d,根处理最有利于以多聚物、氨基酸、糖类、胺类、羧酸为碳源的微生物生长,叶处理最有利于以酚酸为碳源的微生物生长,根系分泌物处理微生物生长情况仅好于对照.处理30、60、90、180 d,根处理最有利于以酚酸为碳源的微生物生长,叶处理最有利于以多聚物、氨基酸为碳源的微生物生长,根系分泌物处理微生物多样性最差,不利于以各类碳源为底物的微生物生长.

英文摘要：

Consecutive monoculture problem has seriously restrained the yield and quality of Rehmannia glutinosa L. Allelopathic autotoxicity is one of the most important reasons for the formation of plants consecutive monoculture problem, so it is particularly important to study the source of allelochemicals （root, leaf and root exudates） of R. glutinosa L. The paper investigated the changes in the functional diversities of microbial flora in soil during different periods after adding different carriers of allelochemicals from R. glutinosa L. （root, leaf and root exudates） to soil. By BIOLOG technology analyzing the growth of microbial flora feeding on phenolic acids and other carbohydrates as carbon sources of every treatments during different periods, the author studied the main source of allelochemicals. BIOLOG analysis results indicated that, when treated for 1 day, the root treatment was most conductive to the growth of the microbial flora feeding on polymers, amino acids, amine, carboxylic acids and carbo-hydrates as carbon sources, the leaf treatment was most conductive to the growth of the microbial flora feeding on phenolic acids as carbon sources, and the root exudates treatment＇s growth of microbial flora in soil was just better than the control. When treated for 30 60 90 180 days, the root treatment was best for the growth of the microbial flora feeding on phenolic acids as carbon sources, the leaf treatment was most beneficial to the growth of microbial flora feeding on polymers, amino acids as carbon sources, while the root exudates treatment was not conductive to the growth of microbial flora feeding on all carbon sources.