中文摘要：

选用热变性法、离子交换层析技术和聚丙烯酰胺凝胶电泳技术（PAGE）小批量制备电泳纯魟鱼肝铁蛋白（Liverferritin of Dasyatisakajei，DALF）和黄牛胰脏铁蛋白（Scalper pancreas ferritin，SPF）．等电聚胶电泳技术指出，DALF和SPF分别显示3和2条不同等电点铁蛋白层析带，推测这一现象与铁蛋白含铁量无关，与各自蛋白壳H和L亚基分布不同且形成不同表面电荷有关．不同等电点且相同含铁量的DALF，表现出不同的释放铁速率，其释放铁全过程均可分为快速和慢速阶段，符合一级反应动力学规律．铁蛋白可通过自身蛋白壳H和L亚基分布和相互作用强度的差异性，产生不同的柔性调节作用，控制不同释放铁的速率．

英文摘要：

Liver ferritin ofDasyatis akajei （DALF） and scalper pancreas ferritin （SPF） with electrophoresis purity were prepared by heat denatured method, ion-exchange chromatography, and polyacrylamide gel electrophoresis （PAGE） in miniature. It was indicated that DALF and SPF showed three and two protein bands with different isoelectric points revealed with a technology of isoelectric point in gel, respectively. We suggested that these phenomena were unconnected with the iron content within the ferritin, but related with differential distribution both H and L subunits including forming different surface charges on the surface of ferritin shell itself. Both DALFs with different isoelectric points indduing same iron content showed different rate of iron release. The kinetic process for whole iron release could be divided into two phases of fast and slow rates, and both phases followed first-law kinetics. It was indicated that the ferritin had ability for controlling different rates of iron release in ferritin using differential characteristics of its subunit distribution and the interaction intensity both H and L subunits for making different flexible regulation on the surface of protein shell itself, respectively.