中文摘要：

小批量制备电泳纯魟鱼肝铁蛋白（liver ferritin of Daryatisakajel，DALF）．在不同反应温度和pH条件下，研究温度和pH对DALF释放铁速率、动力学和级数转换特性的影响．实验结果表明，随着反应温度递增（30-45℃），DALF释放铁的速率明显不同，但DALF释放铁的全过程均呈复杂途径．通过提高反应温度和增加释放铁的速率途径均无法使DALF释放铁的途径由复杂转换为简单．不同pH的反应介质直接影响DALF释放铁的速率，在弱酸介质中，DALF释放铁的速率明显高于在弱碱介质中．在pH5．0条件下，DALF以一级反应动力学的全过程途径释放铁，使释放铁的复杂途径趋于简单化．在生理pH或中性介质条件下，铁蛋白亚基之间相互作用强度起着控释DALF释放铁速率且表现出复杂动力学特性．

英文摘要：

Liver ferritin of Dasyatis akajei （DALF） with electrophoretic purity was prepared in batch. The rate, the kinetics and the law conversion for complete iron release in DALF were revealed by both different reaction temperature and pH, respectively. The experimental results showed that the rates of iron release displayed different evidently though the temperatures were enhanced from 30℃ to 45%2 in the reaction medium. However, this process still exhibited a complex pathway for whole iron release in DALF. Being increasing the rate treated with the reacdve temperature increment, this complex process for iron release would be hardly transferred into simple in DALF. It was well seen that the reaction medium of different pH effected directly on the rate of iron release in DALF, indicating that this rate at acid medium showed higher than that at alkalescence . Under the condition of pH 5. 0, DALF released its iron as first-order law for a whole process, and transfered this complex pathway into simple directly. Under the condition of physiological or neutral medium, the interaction intensity of these subunits in DALF played an important role in controlling iron release and carrying out complex kinetic characteristics.